Chloride intracellular channel 1 (CLIC1) contributes to modulation of cyclic AMP-activated whole cell chloride currents in human bronchial epithelial cells

Liu, Bo; Billington, Charlotte K.; Henry, Amanda P.; Bhaker, Sangita K.; Kheirallah, Alexander K.; Swan, Caroline; Hall, Ian P.

Abstract

Chloride channels are known to play critical physiological roles in many cell types. Here we describe the expression of anion channels using RNA Seq in primary cultures of human Bronchial Epithelial Cells (hBECs). Chloride intracellular channel (CLIC) family members were the most abundant chloride channel transcripts, and CLIC1 showed the highest level of expression. In addition, we characterise the chloride currents in hBECs and determine how inhibition of CLIC1 via pharmacological and molecular approaches impacts these. We demonstrate that CLIC1 is able to modulate cyclic AMP-induced chloride currents and suggest that CLIC1 modulation could be important for chloride homeostasis in this cell type.

Citation

Liu, B., Billington, C. K., Henry, A. P., Bhaker, S. K., Kheirallah, A. K., Swan, C., & Hall, I. P. (in press). Chloride intracellular channel 1 (CLIC1) contributes to modulation of cyclic AMP-activated whole cell chloride currents in human bronchial epithelial cells. Physiological Reports, 6(2), Article e13508. https://doi.org/10.14814/phy2.13508

• Journal Article Type: Article
• Acceptance Date: Oct 26, 2017
• Online Publication Date: Jan 25, 2018
• Deposit Date: Dec 15, 2017
• Publicly Available Date: Jan 25, 2018
• Journal: Physiological Reports
• Electronic ISSN: 2051-817X
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 6
• Issue: 2
• Article Number: e13508
• DOI: https://doi.org/10.14814/phy2.13508
• Keywords: Human bronchial epithelial cells; chloride channels; CLIC1; cyclic AMP
• Public URL: https://nottingham-repository.worktribe.com/output/906608
• Publisher URL: http://physreports.physiology.org/content/6/2/e13508
• Contract Date: Dec 15, 2017