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Chloride intracellular channel 1 (CLIC1) contributes to modulation of cyclic AMP-activated whole cell chloride currents in human bronchial epithelial cells

Liu, Bo; Billington, Charlotte K.; Henry, Amanda P.; Bhaker, Sangita K.; Kheirallah, Alexander K.; Swan, Caroline; Hall, Ian P.

Authors

BO LIU BO.LIU@NOTTINGHAM.AC.UK
Electrophysiologist

Charlotte K. Billington

Amanda P. Henry

Sangita K. Bhaker

Alexander K. Kheirallah

Caroline Swan

IAN HALL ian.hall@nottingham.ac.uk
Professor of Molecular Medicine



Abstract

Chloride channels are known to play critical physiological roles in many cell types. Here we describe the expression of anion channels using RNA Seq in primary cultures of human Bronchial Epithelial Cells (hBECs). Chloride intracellular channel (CLIC) family members were the most abundant chloride channel transcripts, and CLIC1 showed the highest level of expression. In addition, we characterise the chloride currents in hBECs and determine how inhibition of CLIC1 via pharmacological and molecular approaches impacts these. We demonstrate that CLIC1 is able to modulate cyclic AMP-induced chloride currents and suggest that CLIC1 modulation could be important for chloride homeostasis in this cell type.

Citation

Liu, B., Billington, C. K., Henry, A. P., Bhaker, S. K., Kheirallah, A. K., Swan, C., & Hall, I. P. (in press). Chloride intracellular channel 1 (CLIC1) contributes to modulation of cyclic AMP-activated whole cell chloride currents in human bronchial epithelial cells. Physiological Reports, 6(2), https://doi.org/10.14814/phy2.13508

Journal Article Type Article
Acceptance Date Oct 26, 2017
Online Publication Date Jan 25, 2018
Deposit Date Dec 15, 2017
Publicly Available Date Jan 25, 2018
Journal Physiological Reports
Electronic ISSN 2051-817X
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 6
Issue 2
Article Number e13508
DOI https://doi.org/10.14814/phy2.13508
Keywords Human bronchial epithelial cells; chloride channels; CLIC1; cyclic AMP
Public URL http://eprints.nottingham.ac.uk/id/eprint/48775
Publisher URL http://physreports.physiology.org/content/6/2/e13508
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0





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