Effects of hypoxia and hyperoxia on the differential expression of VEGF-A isoforms and receptors in Idiopathic Pulmonary Fibrosis (IPF)

Barratt, Shaney L.; Blythe, Thomas; Ourradi, Khadija; Jarrett, Caroline; Welsh, Gavin I.; Bates, David O.; Millar, Ann B.

Abstract

Dysregulation of VEGF-A bioavailability has been implicated in the development of lung injury/fibrosis, exemplified by Idiopathic Pulmonary Fibrosis (IPF). VEGF-A is a target of the hypoxic response via its translational regulation by HIF-1α. The role of hypoxia and hyperoxia in the development and progression of IPF has not been explored. In normal lung (NF) and IPF-derived fibroblasts (FF) VEGF-Aa protein expression was upregulated by hypoxia, mediated through activation of VEGF-Aa gene transcription. VEGF-A receptors and co-receptors were differentially expressed by hypoxia and hyperoxia. Our data supports a potential role for hypoxia, hyperoxia and VEGF-Aa isoforms as drivers of fibrogenesis.

Citation

Barratt, S. L., Blythe, T., Ourradi, K., Jarrett, C., Welsh, G. I., Bates, D. O., & Millar, A. B. (in press). Effects of hypoxia and hyperoxia on the differential expression of VEGF-A isoforms and receptors in Idiopathic Pulmonary Fibrosis (IPF). Respiratory Research, 19, https://doi.org/10.1186/s12931-017-0711-x

• Journal Article Type: Article
• Acceptance Date: Dec 26, 2017
• Online Publication Date: Jan 15, 2018
• Deposit Date: Mar 5, 2018
• Publicly Available Date: Mar 5, 2018
• Journal: Respiratory Research
• Print ISSN: 1465-9921
• Electronic ISSN: 1465-993X
• Publisher: Springer Verlag
• Peer Reviewed: Peer Reviewed
• Volume: 19
• DOI: https://doi.org/10.1186/s12931-017-0711-x
• Keywords: Interstitial lung disease, Vascular endothelial growth factor, Hypoxia, Idiopathic pulmonary fibrosis
• Public URL: https://nottingham-repository.worktribe.com/output/904549
• Publisher URL: https://respiratory-research.biomedcentral.com/articles/10.1186/s12931-017-0711-x
• Contract Date: Mar 5, 2018