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A minimally invasive optical trapping system to understand cellular interactions at onset of an immune response

Glass, David G.; McAlinden, Niall; Millington, Owain R.; Wright, Amanda J.

Authors

David G. Glass

Niall McAlinden

Owain R. Millington

Amanda J. Wright amanda.wright@nottingham.ac.uk



Abstract

T-cells and antigen presenting cells are an essential part of the adaptive immune response system and how they interact is crucial in how the body effectively fights infection or responds to vaccines. Much of the experimental work studying interaction forces between cells has looked at the average properties of bulk samples of cells or applied microscopy to image the dynamic contact between these cells. In this paper we present a novel optical trapping technique for interrogating the force of this interaction and measuring relative interaction forces at the single-cell level. A triple-spot optical trap is used to directly manipulate the cells of interest without introducing foreign bodies such as beads to the system. The optical trap is used to directly control the initiation of cell-cell contact and, subsequently to terminate the interaction at a defined time point. The laser beam power required to separate immune cell pairs is determined and correlates with the force applied by the optical trap. As proof of concept, the antigen-specific increase in interaction force between a dendritic cell and a specific T-cell is demonstrated. Furthermore, it is demonstrated that this interaction force is completely abrogated when T- cell signalling is blocked. As a result the potential of using optical trapping to interrogate cellular interactions at the single cell level without the need to introduce foreign bodies such as beads is clearly demonstrated.

Journal Article Type Article
Publication Date Dec 8, 2017
Journal PLoS ONE
Electronic ISSN 1932-6203
Publisher Public Library of Science
Peer Reviewed Peer Reviewed
Volume 12
Issue 12
Article Number e0188581
APA6 Citation Glass, D. G., McAlinden, N., Millington, O. R., & Wright, A. J. (2017). A minimally invasive optical trapping system to understand cellular interactions at onset of an immune response. PLoS ONE, 12(12), https://doi.org/10.1371/journal.pone.0188581
DOI https://doi.org/10.1371/journal.pone.0188581
Publisher URL https://doi.org/10.1371/journal.pone.0188581
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0





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