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Performance optimisation of a holographic Fourier domain diffuse correlation spectroscopy instrument

James, Edward; Powell, Samuel; Munro, Peter


Edward James

Peter Munro


We have previously demonstrated a novel interferometric multispeckle Fourier domain diffuse correlation spectroscopy system that makes use of holographic camera-based detection, and which is capable of making in vivo pulsatile flow measurements. In this work, we report on a systematic characterisation of the signal-to-noise ratio performance of our system. This includes demonstration and elimination of laser mode hopping, and correction for the instrument’s modulation transfer function to ensure faithful reconstruction of measured intensity profiles. We also demonstrate a singular value decomposition approach to ensure that spatiotemporally correlated experimental noise sources do not limit optimal signal-to-noise ratio performance. Finally, we present a novel multispeckle denoising algorithm that allows our instrument to achieve a signal-to-noise ratio gain that is equal to the square root of the number of detected speckles, whilst detecting up to ∼1290 speckles in parallel. The signal-to-noise ratio gain of 36 that we report is a significant step toward mitigating the trade-off that exists between signal-to-noise ratio and imaging depth in diffuse correlation spectroscopy.


James, E., Powell, S., & Munro, P. (2022). Performance optimisation of a holographic Fourier domain diffuse correlation spectroscopy instrument. Biomedical Optics Express, 13(7), 3836-3853.

Journal Article Type Article
Acceptance Date Apr 22, 2022
Online Publication Date Jun 9, 2022
Publication Date Jul 1, 2022
Deposit Date Jul 10, 2022
Publicly Available Date Jul 12, 2022
Journal Biomedical Optics Express
Electronic ISSN 2156-7085
Publisher Optical Society of America
Peer Reviewed Peer Reviewed
Volume 13
Issue 7
Pages 3836-3853
Keywords Atomic and Molecular Physics, and Optics; Biotechnology
Public URL
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