Skip to main content

Research Repository

Advanced Search

Photosensitized INA-Labelled protein 1 (PhIL1) is novel component of the inner membrane complex and is required for Plasmodium parasite development

Saini, Ekta; Zeeshan, Mohammad; Brady, Declan; Pandey, Rajan; Kaiser, Gesine; Koreny, Ludek; Kumar, Pradeep; Thakur, Vandana; Tatiya, Shreyansh; Katris, Nicholas J.; Limenitakis, Rebecca Stanway; Kaur, Inderjeet; Green, Judith L.; Bottrill, Andrew R.; Guttery, David S.; Waller, Ross F.; Heussler, Volker; Holder, Anthony A.; Mohmmed, Asif; Malhotra, Pawan; Tewari, Rita


Ekta Saini

Declan Brady

Rajan Pandey

Gesine Kaiser

Ludek Koreny

Pradeep Kumar

Vandana Thakur

Shreyansh Tatiya

Nicholas J. Katris

Rebecca Stanway Limenitakis

Inderjeet Kaur

Judith L. Green

Andrew R. Bottrill

David S. Guttery

Ross F. Waller

Volker Heussler

Anthony A. Holder

Asif Mohmmed

Pawan Malhotra

Professor of Parasite Cell Biology


Plasmodium parasites, the causative agents of malaria, possess a distinctive membranous structure of flattened alveolar vesicles supported by a proteinaceous network, and referred to as the inner membrane complex (IMC). The IMC has a role in actomyosin-mediated motility and host cell invasion. Here, we examine the location, protein interactome and function of PhIL1, an IMC-associated protein on the motile and invasive stages of both human and rodent parasites. We show that PhIL1 is located in the IMC in all three invasive (merozoite, ookinete-, and sporozoite) stages of development, as well as in the male gametocyte and locates both at the apical and basal ends of ookinete and sporozoite stages. Proteins interacting with PhIL1 were identified, showing that PhIL1 was bound to only some proteins present in the glideosome motor complex (GAP50, GAPM1–3) of both P. falciparum and P. berghei. Analysis of PhIL1 function using gene targeting approaches indicated that the protein is required for both asexual and sexual stages of development. In conclusion, we show that PhIL1 is required for development of all zoite stages of Plasmodium and it is part of a novel protein complex with an overall composition overlapping with but different to that of the glideosome.


Saini, E., Zeeshan, M., Brady, D., Pandey, R., Kaiser, G., Koreny, L., …Tewari, R. (in press). Photosensitized INA-Labelled protein 1 (PhIL1) is novel component of the inner membrane complex and is required for Plasmodium parasite development. Scientific Reports, 7(1), Article 15577.

Journal Article Type Article
Acceptance Date Nov 1, 2017
Online Publication Date Nov 14, 2017
Deposit Date Nov 20, 2017
Publicly Available Date Nov 20, 2017
Journal Scientific Reports
Electronic ISSN 2045-2322
Publisher Nature Publishing Group
Peer Reviewed Peer Reviewed
Volume 7
Issue 1
Article Number 15577
Public URL
Publisher URL


s41598-017-15781-z.pdf (1.9 Mb)

Copyright Statement
Copyright information regarding this work can be found at the following address:

You might also like

Downloadable Citations