Gene therapy using plasmid DNA encoding vascular endothelial growth factor 164 and fibroblast growth factor 2 genes for the treatment of horse tendinitis and desmitis: case reports

Kovac, Milomir; Litvin, Yaroslav A.; Aliev, Ruslan O.; Zakirova, Elena Yu; Rutland, Catrin S.; Kiyasov, Andrey; Rizvanov, Albert A.

doi:10.3389/fvets.2017.00168

Gene therapy using plasmid DNA encoding vascular endothelial growth factor 164 and fibroblast growth factor 2 genes for the treatment of horse tendinitis and desmitis: case reports

Kovac, Milomir; Litvin, Yaroslav A.; Aliev, Ruslan O.; Zakirova, Elena Yu; Rutland, Catrin S.; Kiyasov, Andrey; Rizvanov, Albert A.

Authors

Milomir Kovac

Yaroslav A. Litvin

Ruslan O. Aliev

Elena Yu Zakirova

Professor CATRIN RUTLAND CATRIN.RUTLAND@NOTTINGHAM.AC.UK
PROFESSOR OF MOLECULAR MEDICINE

Andrey Kiyasov

Albert A. Rizvanov

Abstract

In this clinical study, for the first time we used the direct gene therapy to restore severe injuries of the suspensory ligament branch and superficial digital flexor tendon in horses (Equus caballus). We injected the plasmid DNA encoding two therapeutic species-specific growth factors: vascular endothelial growth factor 164 and fibroblast growth factor 2 at the site of injury in the suspensory ligament branch and tendon. Treatment effects were evaluated with the use of clinical observation and ultrasound imaging during a period of a few months. We showed that gene therapy used within a period of 2–3 months after the injury resulted in the complete recovery of functions and full restoration of the severely damaged suspensory ligament and superficial digital flexor tendon.

Citation

Kovac, M., Litvin, Y. A., Aliev, R. O., Zakirova, E. Y., Rutland, C. S., Kiyasov, A., & Rizvanov, A. A. (2017). Gene therapy using plasmid DNA encoding vascular endothelial growth factor 164 and fibroblast growth factor 2 genes for the treatment of horse tendinitis and desmitis: case reports. Frontiers in Veterinary Science, 4(168), https://doi.org/10.3389/fvets.2017.00168

Journal Article Type	Article
Acceptance Date	Sep 25, 2017
Publication Date	Oct 10, 2017
Deposit Date	Oct 10, 2017
Publicly Available Date	Oct 10, 2017
Journal	Frontiers in Veterinary Science
Electronic ISSN	2297-1769
Publisher	Frontiers Media
Peer Reviewed	Peer Reviewed
Volume	4
Issue	168
DOI	https://doi.org/10.3389/fvets.2017.00168
Keywords	Gene therapy, Tendon, Suspensory ligament, Horse, Vascular endothelial growth factor, Fibroblast growth factor
Public URL	https://nottingham-repository.worktribe.com/output/887390
Publisher URL	https://www.frontiersin.org/articles/10.3389/fvets.2017.00168/full
Contract Date	Oct 10, 2017