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Gene therapy using plasmid DNA encoding vascular endothelial growth factor 164 and fibroblast growth factor 2 genes for the treatment of horse tendinitis and desmitis: case reports

Kovac, Milomir; Litvin, Yaroslav A.; Aliev, Ruslan O.; Zakirova, Elena Yu; Rutland, Catrin S.; Kiyasov, Andrey; Rizvanov, Albert A.

Authors

Milomir Kovac

Yaroslav A. Litvin

Ruslan O. Aliev

Elena Yu Zakirova

Andrey Kiyasov

Albert A. Rizvanov



Abstract

In this clinical study, for the first time we used the direct gene therapy to restore severe injuries of the suspensory ligament branch and superficial digital flexor tendon in horses (Equus caballus). We injected the plasmid DNA encoding two therapeutic species-specific growth factors: vascular endothelial growth factor 164 and fibroblast growth factor 2 at the site of injury in the suspensory ligament branch and tendon. Treatment effects were evaluated with the use of clinical observation and ultrasound imaging during a period of a few months. We showed that gene therapy used within a period of 2–3 months after the injury resulted in the complete recovery of functions and full restoration of the severely damaged suspensory ligament and superficial digital flexor tendon.

Citation

Kovac, M., Litvin, Y. A., Aliev, R. O., Zakirova, E. Y., Rutland, C. S., Kiyasov, A., & Rizvanov, A. A. (2017). Gene therapy using plasmid DNA encoding vascular endothelial growth factor 164 and fibroblast growth factor 2 genes for the treatment of horse tendinitis and desmitis: case reports. Frontiers in Veterinary Science, 4(168), https://doi.org/10.3389/fvets.2017.00168

Journal Article Type Article
Acceptance Date Sep 25, 2017
Publication Date Oct 10, 2017
Deposit Date Oct 10, 2017
Publicly Available Date Oct 10, 2017
Journal Frontiers in Veterinary Science
Electronic ISSN 2297-1769
Publisher Frontiers Media
Peer Reviewed Peer Reviewed
Volume 4
Issue 168
DOI https://doi.org/10.3389/fvets.2017.00168
Keywords Gene therapy, Tendon, Suspensory ligament, Horse, Vascular endothelial growth factor, Fibroblast
growth factor
Public URL http://eprints.nottingham.ac.uk/id/eprint/47109
Publisher URL https://www.frontiersin.org/articles/10.3389/fvets.2017.00168/full
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0

Files

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Publisher Licence URL
http://creativecommons.org/licenses/by/4.0/

Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0





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