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Measuring and modelling the response of Klebsiella pneumoniae KPC prey to Bdellovibrio bacteriovorus predation, in human serum and defined buffer

Baker, Michelle; Negus, David; Raghunathan, Dhaarini; Radford, Paul; Moore, Christopher; Clark, Gemma; Diggle, Mathew; Tyson, Jess; Twycross, Jamie; Sockett, R. Elizabeth

Measuring and modelling the response of Klebsiella pneumoniae KPC prey to Bdellovibrio bacteriovorus predation, in human serum and defined buffer Thumbnail


Authors

David Negus

Dhaarini Raghunathan

Paul Radford

Christopher Moore

Gemma Clark

Mathew Diggle

Jess Tyson



Abstract

In worldwide conditions of increasingly antibiotic-resistant hospital infections, it is important to research alternative therapies. Bdellovibrio bacteriovorus bacteria naturally prey on Gram-negative pathogens, including antibiotic-resistant strains and so B. bacteriovorus have been proposed as “living antibiotics” to combat antimicrobially-resistant pathogens. Predator-prey interactions are complex and can be altered by environmental components. To be effective B. bacteriovorus predation needs to work in human body fluids such as serum where predation dynamics may differ to that studied in laboratory media. Here we combine mathematical modelling and lab experimentation to investigate the predation of an important carbapenem-resistant human pathogen, Klebsiella pneumoniae, by B. bacteriovorus in human serum versus buffer. We show experimentally that B. bacteriovorus is able to reduce prey numbers in each environment, on different timescales. Our mathematical model captures the underlying dynamics of the experimentation, including an initial predation-delay at the predator-preyserum interface. Our research shows differences between predation in buffer and serum and highlights both the potential and limitations of B. bacteriovorus acting therapeutically against K. pneumoniae in serum, informing future research into the medicinal behaviours and dosing of this living antibacterial.

Citation

Baker, M., Negus, D., Raghunathan, D., Radford, P., Moore, C., Clark, G., …Sockett, R. E. (in press). Measuring and modelling the response of Klebsiella pneumoniae KPC prey to Bdellovibrio bacteriovorus predation, in human serum and defined buffer. Scientific Reports, 7(8329), https://doi.org/10.1038/s41598-017-08060-4

Journal Article Type Article
Acceptance Date Jul 4, 2017
Online Publication Date Aug 21, 2017
Deposit Date Aug 22, 2017
Publicly Available Date Aug 22, 2017
Journal Scientific Reports
Electronic ISSN 2045-2322
Publisher Nature Publishing Group
Peer Reviewed Peer Reviewed
Volume 7
Issue 8329
DOI https://doi.org/10.1038/s41598-017-08060-4
Keywords Bdellovibrio, Pathogen, Serum, Klebsiella, modelling
Public URL https://nottingham-repository.worktribe.com/output/878536
Publisher URL http://www.nature.com/articles/s41598-017-08060-4
Related Public URLs https://www.nature.com/articles/s41598-017-08060-4
Contract Date Aug 22, 2017

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