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The IMPROVE guidelines (Ischaemia Models: Procedural Refinements Of in Vivo Experiments)

Percie du Sert, Nathalie; Alfieri, Alessio; Allan, Stuart M.; Carswell, Hilary V.O.; Deuchar, Graeme A.; Farr, Tracy D.; Flecknell, Paul; Gallagher, Lindsay; Gibson, Claire L.; Haley, Michael J.; Macleod, Malcolm R.; McColl, Barry W.; McCabe, Christopher; Morancho, Anna; Moon, Lawrence D.F.; O’Neill, Michael J.; Pérez de Puig, Isabel; Planas, Anna; Ragan, C. Ian; Rosell, Anna; Roy, Lisa A.; Ryder, Kathryn O.; Simats, Alba; Sena, Emily S.; Sutherland, Brad A.; Tricklebank, Mark D.; Trueman, Rebecca C.; Whitfield, Lucy; Wong, Raymond; Macrae, I. Mhairi

Authors

Nathalie Percie du Sert

Alessio Alfieri

Stuart M. Allan

Hilary V.O. Carswell

Graeme A. Deuchar

Tracy D. Farr

Paul Flecknell

Lindsay Gallagher

Claire L. Gibson

Michael J. Haley

Malcolm R. Macleod

Barry W. McColl

Christopher McCabe

Anna Morancho

Lawrence D.F. Moon

Michael J. O’Neill

Isabel Pérez de Puig

Anna Planas

C. Ian Ragan

Anna Rosell

Lisa A. Roy

Kathryn O. Ryder

Alba Simats

Emily S. Sena

Brad A. Sutherland

Mark D. Tricklebank

Rebecca C. Trueman

Lucy Whitfield

Raymond Wong

I. Mhairi Macrae



Abstract

Most in vivo models of ischaemic stroke target the middle cerebral artery and a spectrum of stroke severities, from mild to substantial, can be achieved. This review describes opportunities to improve the in vivo modelling of ischaemic stroke and animal welfare. It provides a number of recommendations to minimise the level of severity in the most common rodent models of middle cerebral artery occlusion, while sustaining or improving the scientific outcomes. The recommendations cover basic requirements pre-surgery, selecting the most appropriate anaesthetic and analgesic regimen, as well as intraoperative and post-operative care. The aim is to provide support for researchers and animal care staff to refine their procedures and practices, and implement small incremental changes to improve the welfare of the animals used and to answer the scientific question under investigation. All recommendations are recapitulated in a summary poster (see supplementary information).

Journal Article Type Article
Journal Journal of Cerebral Blood Flow & Metabolism
Print ISSN 0271-678X
Electronic ISSN 1559-7016
Publisher SAGE Publications
Peer Reviewed Peer Reviewed
APA6 Citation Percie du Sert, N., Alfieri, A., Allan, S. M., Carswell, H. V., Deuchar, G. A., Farr, T. D., …Macrae, I. M. (in press). The IMPROVE guidelines (Ischaemia Models: Procedural Refinements Of in Vivo Experiments). Journal of Cerebral Blood Flow and Metabolism, https://doi.org/10.1177/0271678X17709185
DOI https://doi.org/10.1177/0271678X17709185
Keywords 3Rs, Animal welfare, Guidelines, Middle cerebral artery occlusion, Stroke
Publisher URL http://journals.sagepub.com/doi/10.1177/0271678X17709185
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0

Files

PercieduSert2017JCBFM.pdf (388 Kb)
PDF

Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0


IMPROVE guidelines poster.pdf (260 Kb)
PDF

Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0





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