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Relaxin-family peptide receptors 1 and 2 are fully functional in the bovine

Dai, Yanzhenzhi; Ivell, Richard; Liu, Xuan; Janowski, Dana; Anand-Ivell, Ravinder

Authors

Yanzhenzhi Dai stxyd4@exmail.nottingham.ac.uk

Richard Ivell richard.ivell@nottingham.ac.uk

Xuan Liu

Dana Janowski

Ravinder Anand-Ivell ravinder.anand-ivell@nottingham.ac.uk



Abstract

In most mammals the peptide hormone relaxin is a key physiological component regulating early pregnancy and birth. However, synteny analysis shows that the gene encoding ovarian relaxin-2 is deleted in cows and sheep. While, these ruminants appear to exhibit a relaxin-like physiology, as in other mammals, until now a molecular understanding of this has been lacking. Cloning and expression analysis of the cognate bovine receptor for relaxin, RXFP1, as well as of the structurally related receptor, RXFP2, in female tissues, shows that these are expressed in a similar way to other mammals. RXFP1 transcripts are found in ovarian theca cells, endometrium, and myometrium, whereas RXFP2 transcripts are expressed in ovarian theca cells, oocytes, as well as in myometrium. Transfection of receptor-expressing gene constructs into HEK293 cells indicates that bovine RXFP1 has a greater EC50 at 10–50 nM for porcine or human relaxin, compared to human RXFP1. For bovine RXFP2, in contrast, the EC50 is

Journal Article Type Article
Publication Date Jun 6, 2017
Journal Frontiers in Physiology
Electronic ISSN 1664-042X
Publisher Frontiers Media
Peer Reviewed Peer Reviewed
Volume 8
Article Number 359
APA6 Citation Dai, Y., Ivell, R., Liu, X., Janowski, D., & Anand-Ivell, R. (2017). Relaxin-family peptide receptors 1 and 2 are fully functional in the bovine. Frontiers in Physiology, 8, https://doi.org/10.3389/fphys.2017.00359
DOI https://doi.org/10.3389/fphys.2017.00359
Keywords INSL3; RXFP1; RXFP2; bovine; endometrium; follicle; relaxin
Publisher URL https://www.frontiersin.org/articles/10.3389/fphys.2017.00359/full
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0





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