Relaxin-family peptide receptors 1 and 2 are fully functional in the bovine

Dai, Yanzhenzhi; Ivell, Richard; Liu, Xuan; Janowski, Dana; Anand-Ivell, Ravinder

doi:10.3389/fphys.2017.00359

Relaxin-family peptide receptors 1 and 2 are fully functional in the bovine

Dai, Yanzhenzhi; Ivell, Richard; Liu, Xuan; Janowski, Dana; Anand-Ivell, Ravinder

Authors

Yanzhenzhi Dai

Richard Ivell

Xuan Liu

Dana Janowski

RAVINDER ANAND-IVELL RAVINDER.ANAND-IVELL@NOTTINGHAM.AC.UK
Associate Professor

Abstract

In most mammals the peptide hormone relaxin is a key physiological component regulating early pregnancy and birth. However, synteny analysis shows that the gene encoding ovarian relaxin-2 is deleted in cows and sheep. While, these ruminants appear to exhibit a relaxin-like physiology, as in other mammals, until now a molecular understanding of this has been lacking. Cloning and expression analysis of the cognate bovine receptor for relaxin, RXFP1, as well as of the structurally related receptor, RXFP2, in female tissues, shows that these are expressed in a similar way to other mammals. RXFP1 transcripts are found in ovarian theca cells, endometrium, and myometrium, whereas RXFP2 transcripts are expressed in ovarian theca cells, oocytes, as well as in myometrium. Transfection of receptor-expressing gene constructs into HEK293 cells indicates that bovine RXFP1 has a greater EC50 at 10–50 nM for porcine or human relaxin, compared to human RXFP1. For bovine RXFP2, in contrast, the EC50 is

Citation

Dai, Y., Ivell, R., Liu, X., Janowski, D., & Anand-Ivell, R. (2017). Relaxin-family peptide receptors 1 and 2 are fully functional in the bovine. Frontiers in Physiology, 8, Article 359. https://doi.org/10.3389/fphys.2017.00359

Journal Article Type	Article
Acceptance Date	May 16, 2017
Publication Date	Jun 6, 2017
Deposit Date	Oct 23, 2017
Publicly Available Date	Oct 23, 2017
Journal	Frontiers in Physiology
Electronic ISSN	1664-042X
Publisher	Frontiers Media
Peer Reviewed	Peer Reviewed
Volume	8
Article Number	359
DOI	https://doi.org/10.3389/fphys.2017.00359
Keywords	INSL3; RXFP1; RXFP2; bovine; endometrium; follicle; relaxin
Public URL	https://nottingham-repository.worktribe.com/output/864757
Publisher URL	https://www.frontiersin.org/articles/10.3389/fphys.2017.00359/full