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Subset-specific expression of toll-like receptors by bovine afferent lymph dendritic cells

Werling, Dirk; Hope, Jayne C.; Siddiqui, Nazneen; Widdison, Stephanie; Russell, Chris; Sopp, Paul; Coffey, Tracey J.

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Authors

Dirk Werling

Jayne C. Hope

Nazneen Siddiqui

Stephanie Widdison

Chris Russell

Paul Sopp

Tracey J. Coffey



Abstract

Within the ruminant system, several possibilities exist to generate dendritic cells migrating out from the tissue into the regional draining lymph nodes as afferent lymph dendritic cells (ALDCs). Here, we analyzed toll-like receptor (TLR) 1–10 mRNA expression by using quantitative real-time PCR in highly purified subsets of bovine ALDC. As TLR expression may be influenced by pathogens or vaccines and their adjuvant, it is necessary to understand what TLRs are expressed in a steady-state system to elucidate specific differences and to potentially optimize targeted vaccines. In this study, we have assessed the TLR expression profiles of the four main bovine ALDC subsets [cDC1 and cDC2 (subsets 2–4)]. We demonstrate differences in TLR expression between the four subsets that may reflect the ability of these cells to respond to different pathogens or to respond to adjuvants.

Citation

Werling, D., Hope, J. C., Siddiqui, N., Widdison, S., Russell, C., Sopp, P., & Coffey, T. J. (2017). Subset-specific expression of toll-like receptors by bovine afferent lymph dendritic cells. Frontiers in Veterinary Science, 4, Article 44. https://doi.org/10.3389/fvets.2017.00044

Journal Article Type Article
Acceptance Date Mar 14, 2017
Publication Date Apr 3, 2017
Deposit Date Apr 4, 2017
Publicly Available Date Apr 4, 2017
Journal Frontiers in Veterinary Science
Electronic ISSN 2297-1769
Publisher Frontiers Media
Peer Reviewed Peer Reviewed
Volume 4
Article Number 44
DOI https://doi.org/10.3389/fvets.2017.00044
Keywords Toll-like receptors, Dendritic cell, Afferent lymph, Dendritic cell subsets, Bovine
Public URL https://nottingham-repository.worktribe.com/output/854721
Publisher URL http://journal.frontiersin.org/article/10.3389/fvets.2017.00044/full
Additional Information This Document is Protected by copyright and was first published by Frontiers. All rights reserved. it is reproduced with permission.
Contract Date Apr 4, 2017

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