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Nanostructured materials for photodynamic therapy: synthesis, characterization and in vitro activity

Alea-Reyes, Maria E.; Rodrigues, Mafalda; Serra, Albert; Mora, Margarita; Sagrista, Maria L.; Gonzalez, Asensio; Duran, Sara; Duch, Marta; Plaza, Jose Antonio; Valles, Elisa; Russell, David A.; Pérez-García, Lluïsa

Authors

Maria E. Alea-Reyes

Mafalda Rodrigues

Albert Serra

Margarita Mora

Maria L. Sagrista

Asensio Gonzalez

Sara Duran

Marta Duch

Jose Antonio Plaza

Elisa Valles

David A. Russell



Abstract

Three nanostructured vehicles are proposed as potential carriers for photosensitizers to be used in photodynamic therapy: spherical nanoparticles, hexahedral microparticles and cylindrical magnetic nanorods. A comparative study of their photodynamic properties was performed, and the influence of their size and the amount of loaded porphyrin was considered to discuss their effects in the observed photodynamic activity. All the vehicles have a gold surface, allowing functionalization with a disulphide-containing porphyrin as the photosensitizer, as well as with a PEG-containing thiol to improve their biocompatibility and water solubility. The activity of the porphyrin loaded in each vehicle was assessed through in vitro photocytotoxicity studies using HeLa cells. A synergic effect for the porphyrin toxicity was observed in all of the vehicles. The zinc-containing porphyrin showed better production of singlet oxygen, and proved more photocytotoxic both in solution and loaded in any of the vehicles. The magnetism of the nanorods allows targeting with a magnetic field, but causes their aggregation, hampering the porphyrin's activity. Microparticles showed lower cell internalization but their bigger size allowed a high porphyrin loading, which translated into high photocytotoxicity. The highest cell internalization and photocytotoxicity was observed for the porphyrin-loaded nanoparticles, suggesting that a smaller size is favored in cell uptake.

Journal Article Type Article
Journal RSC Advances
Print ISSN 2046-2069
Electronic ISSN 2046-2069
Publisher Royal Society of Chemistry
Peer Reviewed Peer Reviewed
Volume 7
APA6 Citation Alea-Reyes, M. E., Rodrigues, M., Serra, A., Mora, M., Sagrista, M. L., Gonzalez, A., …Pérez-García, L. (in press). Nanostructured materials for photodynamic therapy: synthesis, characterization and in vitro activity. RSC Advances, 7, https://doi.org/10.1039/c7ra01569k
DOI https://doi.org/10.1039/c7ra01569k
Publisher URL http://pubs.rsc.org/en/Content/ArticleLanding/2017/RA/C7RA01569K#!divAbstract
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by-nc/4.0

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by-nc/4.0





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