Skip to main content

Research Repository

Advanced Search

ID4 levels dictate the stem cell state in mouse spermatogonia

Helsel, Aileen R.; Yang, Qi-En; Oatley, Melissa; Lord, Tessa; Sablitzky, Fred; Oatley, Jon M.

Authors

Aileen R. Helsel

Qi-En Yang

Melissa Oatley

Tessa Lord

Fred Sablitzky

Jon M. Oatley



Abstract

Spermatogenesis is a classic model of cycling cell lineages that depend on a balance between stem cell self-renewal for continuity and the formation of progenitors as the initial step in the production of differentiated cells. The mechanisms that guide the continuum of spermatogonial stem cell (SSC) to progenitor spermatogonial transition and precise identifiers of subtypes in the process are undefined. Here we used an Id4-eGfp reporter mouse to discover that EGFP intensity is predictive of the subsets, with the ID4-EGFPBright population being mostly, if not purely, SSCs, whereas the ID4-EGFPDim population is in transition to the progenitor state. These subsets are also distinguishable by transcriptome signatures. Moreover, using a conditional overexpression mouse model, we found that transition from the stem cell to the immediate progenitor state requires downregulation of Id4 coincident with a major change in the transcriptome. Collectively, our results demonstrate that the level of ID4 is predictive of stem cell or progenitor capacity in spermatogonia and dictates the interface of transition between the different functional states.

Citation

Helsel, A. R., Yang, Q., Oatley, M., Lord, T., Sablitzky, F., & Oatley, J. M. (in press). ID4 levels dictate the stem cell state in mouse spermatogonia. Development, 144, https://doi.org/10.1242/dev.146928

Journal Article Type Article
Acceptance Date Jan 4, 2017
Online Publication Date Feb 13, 2017
Deposit Date Feb 21, 2017
Publicly Available Date Feb 21, 2017
Journal Development
Print ISSN 0950-1991
Electronic ISSN 1477-9129
Publisher Company of Biologists
Peer Reviewed Peer Reviewed
Volume 144
DOI https://doi.org/10.1242/dev.146928
Keywords ID4, Progenitor, Spermatogonia, Stem cell
Public URL http://eprints.nottingham.ac.uk/id/eprint/40666
Publisher URL http://dev.biologists.org/content/144/4/624
Copyright Statement Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf

Files


Helsel et al.pdf (5.7 Mb)
PDF

Copyright Statement
Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf





You might also like



Downloadable Citations