Skip to main content

Research Repository

Advanced Search

A cross-species gene expression marker-based genetic map and QTL analysis in bambara groundnut

Chai, Hui; Ho, Wai; Graham, Neil; May, Sean; Massawe, Festo; Mayes, Sean

Authors

Hui Chai

Wai Ho

NEIL GRAHAM neil.graham@nottingham.ac.uk
Senior Research Fellow

Festo Massawe

SEAN MAYES SEAN.MAYES@NOTTINGHAM.AC.UK
Associate Professor



Abstract

Bambara groundnut (Vigna subterranea (L.) Verdc.) is an underutilised legume crop, which has long been recognised as a protein-rich and drought-tolerant crop, used extensively in Sub-Saharan Africa. The aim of the study was to identify quantitative trait loci (QTL) involved in agronomic and drought-related traits using an expression marker-based genetic map based on major crop resources developed in soybean. The gene expression markers (GEMs) were generated at the (unmasked) probe-pair level after cross-hybridisation of bambara groundnut leaf RNA to the Affymetrix Soybean Genome GeneChip. A total of 753 markers grouped at an LOD (Logarithm of odds) of three, with 527 markers mapped into linkage groups. From this initial map, a spaced expression marker-based genetic map consisting of 13 linkage groups containing 218 GEMs, spanning 982.7 cM (centimorgan) of the bambara groundnut genome, was developed. Of the QTL detected, 46% were detected in both control and drought treatment populations, suggesting that they are the result of intrinsic trait differences between the parental lines used to construct the cross, with 31% detected in only one of the conditions. The present GEM map in bambara groundnut provides one technically feasible route for the translation of information and resources from major and model plant species to underutilised and resource-poor crops.

Journal Article Type Article
Publication Date Feb 22, 2017
Journal Genes
Electronic ISSN 2073-4425
Publisher MDPI
Peer Reviewed Peer Reviewed
Volume 8
Issue 3
APA6 Citation Chai, H., Ho, W., Graham, N., May, S., Massawe, F., & Mayes, S. (2017). A cross-species gene expression marker-based genetic map and QTL analysis in bambara groundnut. Genes, 8(3), https://doi.org/10.3390/genes8020084
DOI https://doi.org/10.3390/genes8020084
Keywords Affymetrix GeneChip; cross-hybridisation; gene expression markers; quantitative trait loci
Publisher URL http://www.mdpi.com/2073-4425/8/2/84
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0

Files

genes-08-00084-v2.pdf (2.6 Mb)
PDF

Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0





You might also like



Downloadable Citations

;