Skip to main content

Research Repository

Advanced Search

Transcriptomic responses of water buffalo liver to infection with the digenetic fluke Fasciola gigantica

Zhang, Fu-Kai; Zhang, Xiao-Xuan; Elsheikha, Hany M.; He, Jun-Jun; Sheng, Zhao-An; Zheng, Wen-Bin; Ma, Jian-Gang; Huang, Wei-Yi; Guo, Ai-Jiang; Zhu, Xing-Quan

Authors

Fu-Kai Zhang

Xiao-Xuan Zhang

Jun-Jun He

Zhao-An Sheng

Wen-Bin Zheng

Jian-Gang Ma

Wei-Yi Huang

Ai-Jiang Guo

Xing-Quan Zhu



Abstract

Background
Fasciola gigantica, the tropical liver fluke, infects buffaloes in Asian and African countries and causes significant economic losses and poses public health threat in these countries. However, little is known of the transcriptional response of buffaloes to infection with F. gigantica. The objective of the present study was to perform the first transcriptomic analysis of buffalo liver infected by F. gigantica. Understanding the mechanisms that underpin F. gigantica infection in buffaloes will contribute to our ability to control this parasite.
Methods
We challenged buffaloes with 500 viable F. gigantica metacercariae and collected liver samples through a time course at 3, 42 and 70 days post-infection (dpi). Then, we performed gene expression analysis on liver samples using RNA sequencing (RNA-Seq) Illumina technology and confirmed the RNA-Seq data by quantitative RT-PCR analysis.
Results
Totals of 496, 880 and 441 differentially expressed transcripts were identified in the infected livers at 3, 42 and 70 dpi, respectively. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that transcriptional changes in the liver of infected buffaloes evolve over the course of infection. The predominant response of buffaloes to infection was mediated by certain pathways, such as MHC antigen processing and presentation, Toll-like receptor 4 (TLR4), transforming growth factor beta (TGF-β), and the cytochrome P450. Hepatic drug metabolizing enzymes and bile secretion were also affected.
Conclusions
Fasciola gigantica can induce statistically significant and biologically plausible differences in the hepatic gene expression of infected buffaloes. These findings provide new insights into the response of buffaloes to F. gigantica over the course of infection, which may be useful in determining pathways that can modulate host-parasite interaction and thus potentially important for clearance of the parasite.

Citation

Zhang, F., Zhang, X., Elsheikha, H. M., He, J., Sheng, Z., Zheng, W., …Zhu, X. (2017). Transcriptomic responses of water buffalo liver to infection with the digenetic fluke Fasciola gigantica. Parasites and Vectors, 10(1), https://doi.org/10.1186/s13071-017-1990-2

Journal Article Type Article
Acceptance Date Jan 18, 2017
Online Publication Date Feb 1, 2017
Publication Date 2017-12
Deposit Date Feb 6, 2017
Publicly Available Date Feb 6, 2017
Journal Parasites & Vectors
Electronic ISSN 1756-3305
Publisher Springer Verlag
Peer Reviewed Peer Reviewed
Volume 10
Issue 1
Article Number 56
DOI https://doi.org/10.1186/s13071-017-1990-2
Keywords Fasciola gigantica, Immunomodulation, RNA-sequencing, Transcriptome, Water buffalo
Public URL http://eprints.nottingham.ac.uk/id/eprint/40337
Publisher URL https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-017-1990-2
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0

Files


Hany Elsheikha paper_18.01.2017.pdf (2.8 Mb)
PDF

Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0





You might also like



Downloadable Citations