Characterisation of monoclonal antibodies specific for hamster leukocyte differentiation molecules

Rees, Jennifer; Haig, David; Mack, Victoria; Davis, William C.

Abstract

Flow cytometry was used to identify mAbs that recognize conserved epitopes on hamster leukocyte differentiation molecules (hLDM) and also to characterize mAbs developed against hLDM. Initial screening of mAbs developed against LDMs in other species yielded mAbs specific for the major histocompatibility (MHC) II molecule, CD4 and CD18. Screening of sets of mAbs developed against hLDM yielded 22 new mAbs, including additional mAbs to MHC II molecules and mAbs that recognize LDMs expressed on all leukocytes, granulocytes, all lymphocytes, all T cells, a subset of T cells, or on all B cells. Based on comparison of the pattern of expression of LDMs expressed on all hamster leukocytes with the patterns of expression of known LDMs in other species, as detected by flow cytometry (FC), four mAbs are predicted to recognize CD11a, CD44, and CD45. Cross comparison of mAbs specific for a subset of hamster T cells with a cross reactive mAb known to recognize CD4 in mice and one recognising CD8 revealed they recognize CD4. The characterization of these mAbs expands opportunities to use hamsters as an additional model species to investigate the mechanisms of immunopathogenesis of infectious diseases.

Citation

Rees, J., Haig, D., Mack, V., & Davis, W. C. (in press). Characterisation of monoclonal antibodies specific for hamster leukocyte differentiation molecules. Veterinary Immunology and Immunopathology, 183, https://doi.org/10.1016/j.vetimm.2016.12.003

• Journal Article Type: Article
• Acceptance Date: Dec 5, 2016
• Online Publication Date: Dec 6, 2016
• Deposit Date: Jan 11, 2017
• Publicly Available Date: Jan 11, 2017
• Journal: Veterinary Immunology and Immunopathology
• Print ISSN: 0165-2427
• Electronic ISSN: 1873-2534
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 183
• DOI: https://doi.org/10.1016/j.vetimm.2016.12.003
• Keywords: Hamster, Monoclonal antibody, Flow cytometry, CD4, CD8
• Public URL: https://nottingham-repository.worktribe.com/output/836446
• Publisher URL: http://www.sciencedirect.com/science/article/pii/S0165242716303464
• Contract Date: Jan 11, 2017