Skip to main content

Research Repository

Advanced Search

Characterisation of monoclonal antibodies specific for hamster leukocyte differentiation molecules

Rees, Jennifer; Haig, David; Mack, Victoria; Davis, William C.


Jennifer Rees

David Haig

Victoria Mack

William C. Davis


Flow cytometry was used to identify mAbs that recognize conserved epitopes on hamster leukocyte differentiation molecules (hLDM) and also to characterize mAbs developed against hLDM. Initial screening of mAbs developed against LDMs in other species yielded mAbs specific for the major histocompatibility (MHC) II molecule, CD4 and CD18. Screening of sets of mAbs developed against hLDM yielded 22 new mAbs, including additional mAbs to MHC II molecules and mAbs that recognize LDMs expressed on all leukocytes, granulocytes, all lymphocytes, all T cells, a subset of T cells, or on all B cells. Based on comparison of the pattern of expression of LDMs expressed on all hamster leukocytes with the patterns of expression of known LDMs in other species, as detected by flow cytometry (FC), four mAbs are predicted to recognize CD11a, CD44, and CD45. Cross comparison of mAbs specific for a subset of hamster T cells with a cross reactive mAb known to recognize CD4 in mice and one recognising CD8 revealed they recognize CD4. The characterization of these mAbs expands opportunities to use hamsters as an additional model species to investigate the mechanisms of immunopathogenesis of infectious diseases.


Rees, J., Haig, D., Mack, V., & Davis, W. C. (in press). Characterisation of monoclonal antibodies specific for hamster leukocyte differentiation molecules. Veterinary Immunology and Immunopathology, 183,

Journal Article Type Article
Acceptance Date Dec 5, 2016
Online Publication Date Dec 6, 2016
Deposit Date Jan 11, 2017
Publicly Available Date Jan 11, 2017
Journal Veterinary Immunology and Immunopathology
Print ISSN 0165-2427
Electronic ISSN 1873-2534
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 183
Keywords Hamster, Monoclonal antibody, Flow cytometry, CD4, CD8
Public URL
Publisher URL


Hamster.pdf (819 Kb)

Copyright Statement
Copyright information regarding this work can be found at the following address:

Downloadable Citations