Continuous flow biocatalysis: production and in-line purification of amines by immobilised transaminase from Halomonas elongata

Planchestainer, Matteo; Contente, Martina Letizia; Cassidy, Jennifer; Molinari, Francesco; Tamborini, Lucia; Paradisi, Francesca

Abstract

The continuous flow synthesis of a series of amines was successfully achieved by exploiting the enhanced stability and broad substrate scope of an immobilised transaminases from Halomonas elongata (HEWT). A series of substrates were tested in flow reactors and transformed to the corresponding amines in good to excellent yields. The process was implemented with an integrated in-line purification step for the recovery of the pure amines.

Citation

Planchestainer, M., Contente, M. L., Cassidy, J., Molinari, F., Tamborini, L., & Paradisi, F. (2017). Continuous flow biocatalysis: production and in-line purification of amines by immobilised transaminase from Halomonas elongata. Green Chemistry, 19, https://doi.org/10.1039/c6gc01780k

• Journal Article Type: Article
• Acceptance Date: Aug 3, 2016
• Online Publication Date: Aug 3, 2016
• Publication Date: Jan 1, 2017
• Deposit Date: Jan 27, 2017
• Publicly Available Date: Jan 27, 2017
• Journal: Green Chemistry
• Print ISSN: 1463-9262
• Electronic ISSN: 1463-9270
• Publisher: Royal Society of Chemistry
• Peer Reviewed: Peer Reviewed
• Volume: 19
• DOI: https://doi.org/10.1039/c6gc01780k
• Public URL: https://nottingham-repository.worktribe.com/output/830908
• Publisher URL: http://pubs.rsc.org/en/content/articlehtml/2016/gc/c6gc01780k?page=search
• Contract Date: Jan 27, 2017