Long-term CO₂ injection and its impact on near-surface soil microbiology

Gwosdz, Simone; West, Julia M.; Jones, David; Rakoczy, Jana; Green, Kay; Barlow, Tom; Bl�the, Marco; Smith, Karon L.; Steven, Michael D.; Kr�ger, Martin

Abstract

Impacts of long-term CO₂ exposure on environmental processes and microbial populations of near-surface soils are poorly understood. This near-surface long-term CO₂ injection study demonstrated that soil microbiology and geochemistry is influenced more by seasonal parameters than elevated CO₂. Soil samples were taken during a 3-year field experiment including sampling campaigns before, during and after 24 months of continuous CO₂ injection. CO₂ concentrations within CO₂-injected plots increased up to 23% during the injection period. No CO₂ impacts on geochemistry were detected over time. In addition, CO₂ exposed samples did not show significant changes in microbial CO₂ and CH₄ turnover rates compared to reference samples. Likewise, no significant CO₂-induced variations were detected for the abundance of Bacteria, Archaea (16S rDNA) and gene copy numbers of the mcrA gene, Crenarchaeota and amoA gene. The majority (75%–95%) of the bacterial sequences were assigned to five phyla: Firmicutes, Proteobacteria, Actinobacteria, Acidobacteria and Bacteroidetes. The majority of the archaeal sequences (85%–100%) were assigned to the thaumarchaeotal cluster I.1b (soil group). Univariate and multivariate statistical as well as principal component analyses showed no significant CO₂-induced variation. Instead, seasonal impacts especially temperature and precipitation were detected.

Citation

Gwosdz, S., West, J. M., Jones, D., Rakoczy, J., Green, K., Barlow, T., …Krüger, M. (2016). Long-term CO₂ injection and its impact on near-surface soil microbiology. FEMS Microbiology Ecology, 92(12), Article fiw193. https://doi.org/10.1093/femsec/fiw193

• Journal Article Type: Article
• Acceptance Date: Aug 29, 2016
• Online Publication Date: Sep 8, 2016
• Publication Date: Dec 1, 2016
• Deposit Date: Nov 6, 2017
• Journal: FEMS Microbiology Ecology
• Print ISSN: 0168-6496
• Electronic ISSN: 1574-6941
• Publisher: Oxford University Press
• Peer Reviewed: Peer Reviewed
• Volume: 92
• Issue: 12
• Article Number: fiw193
• DOI: https://doi.org/10.1093/femsec/fiw193
• Keywords: CCS; CO₂-leakage; Bacteria; Archaea; qPCR; pyrosequencing
• Public URL: https://nottingham-repository.worktribe.com/output/825465
• Publisher URL: https://academic.oup.com/femsec/article/92/12/fiw193/2570443
• Contract Date: Nov 6, 2017