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The family-specific α4-helix of the kinesin-13, MCAK, is critical to microtubule end recognition

Patel, Jennifer T.; Belsham, Hannah R.; Rathbone, Alexandra J.; Wickstead, Bill; Gell, Christopher; Friel, Claire T.

Authors

Jennifer T. Patel

Hannah R. Belsham

Alexandra J. Rathbone

Christopher Gell



Abstract

Kinesins that influence the dynamics of microtubule growth and shrinkage require the ability to distinguish between the microtubule end and the microtubule lattice. The microtubule depolymerizing kinesin MCAK has been shown to specifically recognize the microtubule end. This ability is key to the action of MCAK in regulating microtubule dynamics. We show that the a4-helix of the motor domain is crucial to microtubule end recognition. Mutation of the residues K524, E525 and R528, which are located in the C-terminal half of the a4-helix, specifically disrupts the ability of MCAK to recognize the microtubule end. Mutation of these residues, which are conserved in the kinesin-13 family and discriminate members of this family from translocating kinesins, impairs the ability of MCAK to discriminate between the microtubule lattice and the microtubule end.

Citation

Patel, J. T., Belsham, H. R., Rathbone, A. J., Wickstead, B., Gell, C., & Friel, C. T. (2016). The family-specific α4-helix of the kinesin-13, MCAK, is critical to microtubule end recognition. Open Biology, 6(10), https://doi.org/10.1098/rsob.160223

Journal Article Type Article
Acceptance Date Sep 10, 2016
Online Publication Date Oct 1, 2016
Publication Date 2016-10
Deposit Date Oct 17, 2016
Publicly Available Date Oct 17, 2016
Journal Open Biology
Electronic ISSN 2046-2441
Publisher Royal Society, The
Peer Reviewed Peer Reviewed
Volume 6
Issue 10
Article Number 160223
DOI https://doi.org/10.1098/rsob.160223
Keywords MCAK; kinesin-13; microtubule; depolymerization; ATP turnover; microtubule end recognition
Public URL http://eprints.nottingham.ac.uk/id/eprint/37614
Publisher URL http://rsob.royalsocietypublishing.org/content/6/10/160223
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0





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