Skip to main content

Research Repository

Advanced Search

Clinicopathological and prognostic significance of mitogen-activated protein kinases (MAPK) in breast cancers

Ahmad, Dena Akram Jerjees; Negm, Ola H.; Layth Alabdullah, M.; Mirza, Sameer; Hamed, Mohamed R.; Band, Vimla; Green, Andrew R.; Ellis, Ian O.; Rakha, Emad

Authors

Dena Akram Jerjees Ahmad

OLA NEGM ola.negm@nottingham.ac.uk
Teaching Associate and Problembased Learning Facilitator

M. Layth Alabdullah

Sameer Mirza

Mohamed R. Hamed

Vimla Band

EMAD RAKHA Emad.Rakha@nottingham.ac.uk
Professor of Breast Cancer Pathology



Abstract

Background Mitogen-activated protein kinases (MAPKs) are signalling transduction molecules that have different functions and diverse behaviour in cancer. In breast cancer, MAPK is related to oestrogen receptor (ER) and HER2.
Methods Protein expression of a large panel of MAPKs (JNK1/2, ERK, p38, C-JUN and ATF2 including phosphorylated forms) were assessed immunohistochemically in a large (n = 1400) and well-characterised breast cancer series prepared as tissue microarray. Moreover, reverse phase protein array was applied to quantify protein expression of MAPKs in six breast cancer cell lines with different phenotypes including HER2-transfected cells.
Results MAPKs expression was associated with clinicopathological variables characteristic of good prognosis. These associations were most significant in the whole series and in the ER? subgroup compared to other BC classes. Most of MAPKs showed a positive association with ER, BCL2 and better outcome and were negatively associated with the proliferation marker Ki67 and p53. Association of MAPK with HER2 was mainly seen in the ER- subgroup. Reverse phase protein array confirmed immunohistochemistry results and revealed differential expression of MAPK proteins in ER? and ER- cell lines.
Conclusions MAPKs are associated with good prognosis and their expression is mainly related to ER. Studying a large panel rather than individual biomarkers may provide improved understanding of the pathway

Journal Article Type Article
Publication Date 2016-10
Journal Breast Cancer Research and Treatment
Print ISSN 0167-6806
Electronic ISSN 1573-7217
Publisher Springer Verlag
Peer Reviewed Peer Reviewed
Volume 159
Issue 3
Pages 457-467
APA6 Citation Ahmad, D. A. J., Negm, O. H., Layth Alabdullah, M., Mirza, S., Hamed, M. R., Band, V., …Rakha, E. (2016). Clinicopathological and prognostic significance of mitogen-activated protein kinases (MAPK) in breast cancers. Breast Cancer Research and Treatment, 159(3), 457-467. https://doi.org/10.1007/s10549-016-3967-9
DOI https://doi.org/10.1007/s10549-016-3967-9
Keywords MAPK signalling pathways; reverse phase protein microarray (RPPA); breast cancer
Publisher URL http://link.springer.com/article/10.1007%2Fs10549-016-3967-9
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0

Files

art%3A10.1007%2Fs10549-016-3967-9 - Springer.pdf (969 Kb)
PDF

Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0







You might also like



Downloadable Citations

;