IL-36 receptor is expressed by human blood and intestinal T lymphocytes and is dose–dependently activated via IL-36β and induces CD4+ lymphocyte proliferation

Penha, Rafael; Higgins, John; Mutamba, Shilla; Barrow, Paul; Mahida, Yashwant R.; Foster, Neil

Abstract

We show that IL-36R is expressed by T (CD4+ and CD8+) and B (CD19+) lymphocytes in human blood and also by CD4+ T lymphocytes in the intestinal lamina propria. IL-36R protein was mostly stored in the cytoplasm of CD4 lymphocytes and B cells, during steady state conditions and the greatest expression of IL-36R mRNA was measured in CD4+ (T helper) lymphocytes. IL-36 β, which functions via IL-36R induced rapid and significant (P < 0.05) proliferation of CD4+ lymphocytes, within 48 h. IL-36R expression was also maintained on the surface of circulating CD4+ lymphocytes which enter the intestinal lamina propria.

In conclusion our study is the first to show that (1) all human blood lymphocytes express IL-36R; (2) IL-36R expression is maintained by circulating CD4+ lymphocytes which enter the intestinal lamina propria and (3) IL-36R/IL-36 β induces rapid CD4 lymphocyte proliferation. The possible significance of these results in the context of human disease is discussed.

Citation

Penha, R., Higgins, J., Mutamba, S., Barrow, P., Mahida, Y. R., & Foster, N. (2016). IL-36 receptor is expressed by human blood and intestinal T lymphocytes and is dose–dependently activated via IL-36β and induces CD4+ lymphocyte proliferation. Cytokine, 85, https://doi.org/10.1016/j.cyto.2016.05.023

• Journal Article Type: Article
• Acceptance Date: May 24, 2016
• Online Publication Date: Jun 4, 2016
• Publication Date: Sep 30, 2016
• Deposit Date: Mar 27, 2017
• Publicly Available Date: Mar 27, 2017
• Journal: Cytokine
• Print ISSN: 1043-4666
• Electronic ISSN: 1096-0023
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 85
• DOI: https://doi.org/10.1016/j.cyto.2016.05.023
• Keywords: IL-36; IL-36R; Human; Lymphocyte
• Public URL: https://nottingham-repository.worktribe.com/output/808595
• Publisher URL: https://doi.org/10.1016/j.cyto.2016.05.023
• Contract Date: Mar 27, 2017