Allelic diversity of S‑RNase alleles in diploid potato species

Dzidzienyo, Daniel Kwadjo; Bryan, Glenn J.; Wilde, Gail; Robbins, Timothy P.

Abstract

S-ribonucleases (S-RNases) control the pistil specificity of the self-incompatibility (SI) response in the genus Solanum and several other members of the Solanaceae. The nucleotide sequences of S-RNases corresponding to a large number of S-alleles or S-haplotypes have been characterised. However, surprisingly few S-RNase sequences are available for potato species. The identification of new S-alleles in diploid potato species is desirable as these stocks are important sources of traits such as biotic and abiotic resistance. S-RNase sequences are reported here from three distinct diploid types of potato: cultivated Solanum tuberosum Group Phureja, S. tuberosum Group Stenotomum, and the wild species Solanum okadae. Partial S-RNase sequences were obtained from pistil RNA by RT-PCR or 3’RACE (Rapid Amplification of cDNA Ends) using a degenerate primer. Full length sequences were obtained for two alleles by 5’RACE. Database searches with these sequences, identified sixteen S-RNases in total, all of which are novel. The sequence analysis revealed all the expected features of functional S-RNases. Phylogenetic analysis with selected published S-RNase and S-like-RNase sequences from the Solanaceae revealed extensive trans-generic evolution of the S-RNases and a clear distinction from S-like-RNases. Pollination tests were used to confirm the self-incompatibility status and cross-compatibility relationships of the S. okadae accessions. All the S. okadae accessions were found to be self-incompatible as expected with crosses amongst them exhibiting both cross-compatibility and semi-compatibility consistent with the S-genotypes determined from the S-RNase sequence data. The progeny analysis of four semi-compatible crosses examined by allele-specific PCR provided further confirmation that these are functional S-RNases.

Citation

Dzidzienyo, D. K., Bryan, G. J., Wilde, G., & Robbins, T. P. (2016). Allelic diversity of S‑RNase alleles in diploid potato species. TAG Theoretical and Applied Genetics, 129(10), 1985–2001. https://doi.org/10.1007/s00122-016-2754-7

• Journal Article Type: Article
• Acceptance Date: Jul 15, 2016
• Online Publication Date: Aug 6, 2016
• Publication Date: Oct 1, 2016
• Deposit Date: Aug 5, 2016
• Publicly Available Date: Aug 6, 2016
• Journal: Theoretical and Applied Genetics
• Print ISSN: 0040-5752
• Electronic ISSN: 1432-2242
• Publisher: Springer Verlag
• Peer Reviewed: Peer Reviewed
• Volume: 129
• Issue: 10
• Pages: 1985–2001
• DOI: https://doi.org/10.1007/s00122-016-2754-7
• Public URL: https://nottingham-repository.worktribe.com/output/807847
• Publisher URL: http://link.springer.com/article/10.1007%2Fs00122-016-2754-7
• Contract Date: Aug 5, 2016