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Use of a new proximity assay (NanoBRET) to investigate the ligand-binding characteristics of three fluorescent ligands to the humanβ1-adrenoceptor expressed in HEK-293 cells

Soave, Mark; Stoddart, Leigh A.; Brown, Alastair; Woolard, Jeanette; Hill, Stephen J.

Use of a new proximity assay (NanoBRET) to investigate the ligand-binding characteristics of three fluorescent ligands to the humanβ1-adrenoceptor expressed in HEK-293 cells Thumbnail


Authors

Mark Soave

Leigh A. Stoddart

Alastair Brown

JEANETTE WOOLARD Jeanette.Woolard@nottingham.ac.uk
Professor of Cardiovascular Physiology and Pharmacology

STEPHEN HILL STEVE.HILL@NOTTINGHAM.AC.UK
Professor of Molecular Pharmacology



Abstract

Previous research has indicated that allosteric interactions across the dimer interface of β1-adrenoceptors may be responsible for a secondary low affinity binding conformation. Here we have investigated the potential for probe dependence, in the determination of antagonist pKi values at the human β1-adenoceptor, which may result from such allosterism interactions. Three fluorescent β1-adrenoceptor ligands were used to investigate this using bioluminescence energy transfer (BRET) between the receptor-bound fluorescent ligand and the N-terminal NanoLuc tag of a human β1-adrenoceptor expressed in HEK 293 cells (NanoBRET). This proximity assay showed high affinity specific binding to the NanoLuc-β1-adrenoceptor with each of the three fluorescent ligands yielding KD values of 87.1 ± 10nM (n=8), 38.1 ± 12nM (n=7), 13.4 ± 2nM (n=14) for propranolol-Peg8-BY630, propranolol-3(Ala-Ala)-BY630 and CGP-12177- TMR respectively. Parallel radioligand-binding studies with 3H-CGP12177 and TIRF microscopy, to monitor NanoLuc bioluminescence, confirmed a high cell surface expression of the NanoLuc- 31-adrenoceptor in HEK 293 cells (circa 1500 fmol.mg protein-1). Following a 1h incubation with fluorescent ligands and β1-adrenoceptor competing antagonists, there were significant differences (p < 0.001) in the pKi values obtained for CGP20712a and CGP 12177 with the different fluorescent ligands and 3H-CGP 12177. However, increasing the incubation time to 2h removed these significant differences. The data obtained show that the NanoBRET assay can be applied successfully to study ligand-receptor interactions at the human β1-adrenoceptor. However, the study also emphasizes the importance of ensuring that both the fluorescent and competing ligands are in true equilibrium before interpretations regarding probe dependence can be made.

Citation

Soave, M., Stoddart, L. A., Brown, A., Woolard, J., & Hill, S. J. (2016). Use of a new proximity assay (NanoBRET) to investigate the ligand-binding characteristics of three fluorescent ligands to the humanβ1-adrenoceptor expressed in HEK-293 cells. Pharmacology Research and Perspectives, 4(5), Article e00250. https://doi.org/10.1002/prp2.250

Journal Article Type Article
Acceptance Date Jul 1, 2016
Online Publication Date Aug 8, 2016
Publication Date 2016-10
Deposit Date Jul 18, 2016
Publicly Available Date Aug 8, 2016
Journal Pharmacology Research & Perspectives
Electronic ISSN 2052-1707
Publisher Wiley Open Access
Peer Reviewed Peer Reviewed
Volume 4
Issue 5
Article Number e00250
DOI https://doi.org/10.1002/prp2.250
Keywords Bioluminescence energy transfer; Ligand-binding; 3-adrenoceptors; Probe dependence
Public URL https://nottingham-repository.worktribe.com/output/806356
Publisher URL http://onlinelibrary.wiley.com/doi/10.1002/prp2.250/abstract
Contract Date Jul 18, 2016

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