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Airway and Parenchymal Strains during Bronchoconstriction in the Precision Cut Lung Slice

Hiorns, Jonathan E.; Bidan, C�cile M.; Jensen, Oliver E.; Gosens, Reinoud; Kistemaker, Loes E.M.; Fredberg, Jeffrey J.; Butler, Jim P.; Krishnan, Ramaswamy; Brook, Bindi S.

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Authors

Jonathan E. Hiorns

C�cile M. Bidan

Oliver E. Jensen

Reinoud Gosens

Loes E.M. Kistemaker

Jeffrey J. Fredberg

Jim P. Butler

Ramaswamy Krishnan

BINDI BROOK BINDI.BROOK@NOTTINGHAM.AC.UK
Professor of Mathematical Medicine and Biology



Abstract

The precision-cut lung slice (PCLS) is a powerful tool for studying airway reactivity, but biomechanical measurements to date have largely focused on changes in airway caliber. Here we describe an image processing tool that reveals the associated spatio-temporal changes in airway and parenchymal strains. Displacements of sub-regions within the PCLS are tracked in phase-contrast movies acquired after addition of contractile and relaxing drugs. From displacement maps, strains are determined across the entire PCLS or along user-specified directions. In a representative mouse PCLS challenged with 10−4M methacholine, as lumen area decreased, compressive circumferential strains were highest in the 50 μm closest to the airway lumen while expansive radial strains were highest in the region 50–100 μm from the lumen. However, at any given distance from the airway the strain distribution varied substantially in the vicinity of neighboring small airways and blood vessels. Upon challenge with the relaxant agonist chloroquine, although most strains disappeared, residual positive strains remained a long time after addition of chloroquine, predominantly in the radial direction. Taken together, these findings establish strain mapping as a new tool to elucidate local dynamic mechanical events within the constricting airway and its supporting parenchyma.

Citation

Hiorns, J. E., Bidan, C. M., Jensen, O. E., Gosens, R., Kistemaker, L. E., Fredberg, J. J., …Brook, B. S. (2016). Airway and Parenchymal Strains during Bronchoconstriction in the Precision Cut Lung Slice. Frontiers in Physiology, 7(JUL), Article 309. https://doi.org/10.3389/fphys.2016.00309

Journal Article Type Article
Acceptance Date Jul 7, 2016
Online Publication Date Jul 21, 2016
Publication Date Jul 21, 2016
Deposit Date Jul 3, 2017
Publicly Available Date Jul 3, 2017
Journal Frontiers in Physiology
Electronic ISSN 1664-042X
Publisher Frontiers Media
Peer Reviewed Peer Reviewed
Volume 7
Issue JUL
Article Number 309
DOI https://doi.org/10.3389/fphys.2016.00309
Keywords Airway smooth muscle, Contraction, PCLS, Displacements, Radial strain, Circumferential strain
Public URL https://nottingham-repository.worktribe.com/output/800019
Publisher URL http://journal.frontiersin.org/article/10.3389/fphys.2016.00309/full
Additional Information This Document is Protected by copyright and was first published by Frontiers. All rights reserved. it is reproduced with permission.

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