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Real-time selective sequencing using nanopore technology

Loose, Matthew; Malla, Sunir; Stout, Michael

Authors

MATTHEW LOOSE matt.loose@nottingham.ac.uk
Professor of Developmental and Computational Biology

Sunir Malla

Michael Stout



Abstract

The Oxford Nanopore Technologies MinION sequencer enables the selection of specific DNA molecules for sequencing by reversing the driving voltage across individual nanopores. To directly select molecules for sequencing, we used dynamic time warping to match reads to reference sequences. We demonstrate our open-source Read Until software in real-time selective sequencing of regions within small genomes, individual amplicon enrichment and normalization of an amplicon set.

Journal Article Type Article
Journal Nature Methods
Print ISSN 1548-7091
Electronic ISSN 1548-7105
Publisher Nature Publishing Group
Peer Reviewed Peer Reviewed
Volume 13
Issue 9
APA6 Citation Loose, M., Malla, S., & Stout, M. (in press). Real-time selective sequencing using nanopore technology. Nature Methods, 13(9), https://doi.org/10.1038/nmeth.3930
DOI https://doi.org/10.1038/nmeth.3930
Publisher URL http://www.nature.com/nmeth/journal/v13/n9/full/nmeth.3930.html
Copyright Statement Copyright information regarding this work can be found at the following address: http://eprints.nottingh.../end_user_agreement.pdf

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf





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