MATTHEW LOOSE matt.loose@nottingham.ac.uk
Professor of Developmental and Computational Biology
Real-time selective sequencing using nanopore technology
Loose, Matthew; Malla, Sunir; Stout, Michael
Authors
Sunir Malla
Michael Stout
Abstract
The Oxford Nanopore Technologies MinION sequencer enables the selection of specific DNA molecules for sequencing by reversing the driving voltage across individual nanopores. To directly select molecules for sequencing, we used dynamic time warping to match reads to reference sequences. We demonstrate our open-source Read Until software in real-time selective sequencing of regions within small genomes, individual amplicon enrichment and normalization of an amplicon set.
Citation
Loose, M., Malla, S., & Stout, M. (in press). Real-time selective sequencing using nanopore technology. Nature Methods, 13(9), https://doi.org/10.1038/nmeth.3930
Journal Article Type | Article |
---|---|
Acceptance Date | Jun 22, 2016 |
Online Publication Date | Jul 25, 2016 |
Deposit Date | Oct 19, 2016 |
Publicly Available Date | Oct 19, 2016 |
Journal | Nature Methods |
Print ISSN | 1548-7091 |
Electronic ISSN | 1548-7105 |
Publisher | Nature Publishing Group |
Peer Reviewed | Peer Reviewed |
Volume | 13 |
Issue | 9 |
DOI | https://doi.org/10.1038/nmeth.3930 |
Public URL | https://nottingham-repository.worktribe.com/output/799641 |
Publisher URL | http://www.nature.com/nmeth/journal/v13/n9/full/nmeth.3930.html |
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