Early epigenetic reprogramming in fertilized, cloned, and parthenogenetic embryos

Sepulveda-Rincon, Lessly P.; Solanas, Edgar del Llano; Serrano-Revuelta, Elisa; Ruddick, Lydia; Maalouf, Walid E.; Beaujean, Nathalie

Abstract

Despite ongoing research in a number of species, the efficiency of embryo production by nuclear transfer remains low. Incomplete epigenetic reprogramming of the nucleus introduced in the recipient oocyte is one factor proposed to limit the success of this technique. Nonetheless, knowledge of reprogramming factors has increased—thanks to comparative studies on reprogramming of the paternal genome brought by sperm on fertilization—and will be reviewed here. Another valuable model of reprogramming is the one obtained in the absence of sperm fertilization through artificial activation—the parthenote—and will also be introduced. Altogether the objective of this review is to have a better understanding on the mechanisms responsible for the resistance to reprogramming, not only because it could improve embryonic development but also as it could benefit therapeutic reprogramming research.

Citation

Sepulveda-Rincon, L. P., Solanas, E. D. L., Serrano-Revuelta, E., Ruddick, L., Maalouf, W. E., & Beaujean, N. (2016). Early epigenetic reprogramming in fertilized, cloned, and parthenogenetic embryos. Theriogenology, 86(1), 91-98. https://doi.org/10.1016/j.theriogenology.2016.04.022

• Journal Article Type: Article
• Acceptance Date: Mar 14, 2016
• Online Publication Date: Apr 21, 2016
• Publication Date: Jul 1, 2016
• Deposit Date: Feb 7, 2017
• Publicly Available Date: Feb 7, 2017
• Journal: Theriogenology
• Print ISSN: 0093-691X
• Electronic ISSN: 1879-3231
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 86
• Issue: 1
• Pages: 91-98
• DOI: https://doi.org/10.1016/j.theriogenology.2016.04.022
• Keywords: Oocyte; Nuclear transfer; Embryonic genome activation; Histones posttranslational modifications; DNA methylation
• Public URL: https://nottingham-repository.worktribe.com/output/792423
• Publisher URL: http://dx.doi.org/10.1016/j.theriogenology.2016.04.022