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A protease-based biosensor for the detection of schistosome cercariae

Webb, A.J.; Kelwick, R.; Doenhoff, M.J.; Kylilis, N.; MacDonald, J.T.; Wen, K.Y.; McKeown, C.; Baldwin, G.; Ellis, T.; Jensen, K.; Freemont, P.S.

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Authors

A.J. Webb

R. Kelwick

M.J. Doenhoff

N. Kylilis

J.T. MacDonald

K.Y. Wen

C. McKeown

G. Baldwin

T. Ellis

K. Jensen

P.S. Freemont



Abstract

Parasitic diseases affect millions of people worldwide, causing debilitating illnesses and death. Rapid and cost-effective approaches to detect parasites are needed, especially in resource-limited settings. A common signature of parasitic diseases is the release of specific proteases by the parasites at multiple stages during their life cycles. To this end, we engineered several modular Escherichia coli and Bacillus subtilis whole-cell-based biosensors which incorporate an interchangeable protease recognition motif into their designs. Herein, we describe how several of our engineered biosensors have been applied to detect the presence and activity of elastase, an enzyme released by the cercarial larvae stage of Schistosoma mansoni. Collectively, S. mansoni and several other schistosomes are responsible for the infection of an estimated 200 million people worldwide. Since our biosensors are maintained in lyophilised cells, they could be applied for the detection of S. mansoni and other parasites in settings without reliable cold chain access.

Citation

Webb, A., Kelwick, R., Doenhoff, M., Kylilis, N., MacDonald, J., Wen, K., …Freemont, P. (2016). A protease-based biosensor for the detection of schistosome cercariae. Scientific Reports, 6, Article 24725. https://doi.org/10.1038/srep24725

Journal Article Type Article
Acceptance Date Mar 30, 2016
Online Publication Date Apr 19, 2016
Publication Date Apr 19, 2016
Deposit Date Jul 6, 2016
Publicly Available Date Jul 6, 2016
Journal Scientific Reports
Electronic ISSN 2045-2322
Publisher Nature Publishing Group
Peer Reviewed Peer Reviewed
Volume 6
Article Number 24725
DOI https://doi.org/10.1038/srep24725
Public URL https://nottingham-repository.worktribe.com/output/784677
Publisher URL http://dx.doi.org/10.1038/srep24725
Contract Date Jul 6, 2016

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