Three-dimensional ex vivo co-culture models of the leukaemic bone marrow niche for functional drug testing

Dhami, Sukhraj Pal S.; Kappala, Shanthi S.; Thompson, Alexander; Szegezdi, Eva

Abstract

Acute myeloid leukaemia (AML) is a hierarchically structured malignancy in which aberrant leukemic stem cells drive the production of leukaemic blast cell clones. AML cells strictly depend on the bone marrow microenvironment (BMM) in which they reside. Classical AML cell cultures fail to mimic the BMM and, therefore, drug discovery studies are dominated by in vivo models. However, animal models are time consuming, labour intensive, provide limited mechanistic insight, and are unsuited for high throughput studies, necessitating the development of novel AML models. The evolving ex vivo BMM mimicking culture systems aim to fill this gap, with increasing success. Here, we discuss how AML microenvironment co-culture models advance our understanding of this disease, and highlight their future potential for translational AML research.

Citation

Dhami, S. P. S., Kappala, S. S., Thompson, A., & Szegezdi, E. (in press). Three-dimensional ex vivo co-culture models of the leukaemic bone marrow niche for functional drug testing. Drug Discovery Today, 21(9), https://doi.org/10.1016/j.drudis.2016.04.019

• Journal Article Type: Article
• Acceptance Date: Apr 22, 2016
• Online Publication Date: Apr 27, 2016
• Deposit Date: May 9, 2017
• Journal: Drug Discovery Today
• Print ISSN: 1359-6446
• Electronic ISSN: 1878-5832
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 21
• Issue: 9
• DOI: https://doi.org/10.1016/j.drudis.2016.04.019
• Public URL: https://nottingham-repository.worktribe.com/output/783463
• Publisher URL: http://www.sciencedirect.com/science/article/pii/S1359644616301246
• Contract Date: May 9, 2017