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Three-dimensional ex vivo co-culture models of the leukaemic bone marrow niche for functional drug testing

Dhami, Sukhraj Pal S.; Kappala, Shanthi S.; Thompson, Alexander; Szegezdi, Eva

Authors

Sukhraj Pal S. Dhami

Shanthi S. Kappala

Eva Szegezdi



Abstract

Acute myeloid leukaemia (AML) is a hierarchically structured malignancy in which aberrant leukemic stem cells drive the production of leukaemic blast cell clones. AML cells strictly depend on the bone marrow microenvironment (BMM) in which they reside. Classical AML cell cultures fail to mimic the BMM and, therefore, drug discovery studies are dominated by in vivo models. However, animal models are time consuming, labour intensive, provide limited mechanistic insight, and are unsuited for high throughput studies, necessitating the development of novel AML models. The evolving ex vivo BMM mimicking culture systems aim to fill this gap, with increasing success. Here, we discuss how AML microenvironment co-culture models advance our understanding of this disease, and highlight their future potential for translational AML research.

Citation

Dhami, S. P. S., Kappala, S. S., Thompson, A., & Szegezdi, E. (in press). Three-dimensional ex vivo co-culture models of the leukaemic bone marrow niche for functional drug testing. Drug Discovery Today, 21(9), doi:10.1016/j.drudis.2016.04.019

Journal Article Type Article
Acceptance Date Apr 22, 2016
Online Publication Date Apr 27, 2016
Deposit Date May 9, 2017
Journal Drug Discovery Today
Print ISSN 1359-6446
Electronic ISSN 1878-5832
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 21
Issue 9
DOI https://doi.org/10.1016/j.drudis.2016.04.019
Public URL http://eprints.nottingham.ac.uk/id/eprint/42608
Publisher URL http://www.sciencedirect.com/science/article/pii/S1359644616301246
Copyright Statement Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf