PHILIP ATHERTON philip.atherton@nottingham.ac.uk
Professor of Clinical, metabolic & Molecular Physiology
Enriching a protein drink with leucine augments muscle protein synthesis after resistance exercise in young and older men
Atherton, Philip J.; Kumar, Vinod; Selby, Anna L.; Rankin, Debbie; Hildebrandt, Wulf; Phillips, Bethan E.; Williams, John P.; Hiscock, Natalie; Smith, Kenneth
Authors
Vinod Kumar
Anna L. Selby
Debbie Rankin
Wulf Hildebrandt
BETH PHILLIPS beth.phillips@nottingham.ac.uk
Professor of Translational Physiology
JOHN WILLIAMS john.williams7@nottingham.ac.uk
Clinical Associate Professor
Natalie Hiscock
KENNETH SMITH KEN.SMITH@NOTTINGHAM.AC.UK
Professor of Metabolic Mass Spectrometry
Abstract
© 2016 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism Maximizing anabolic responses to feeding and exercise is crucial for muscle maintenance and adaptation to exercise training. We hypothesized that enriching a protein drink with leucine would improve anabolic responses to resistance exercise (RE: 6×8 knee-extension repetitions at 75% of 1-RM) in both young and older adults. Groups (n=9) of young (24±6y, BMI 23±2kgm−2) and older men (70±5y, BMI 25±2kgm−2) were randomized to either: (i) RE followed by Slim-Fast Optima (SFO 10g PRO; 24g CHO) with 4.2g of leucine (LEU) or, (ii) RE+SFO with 4.2g of alanine (ALA; isonitrogenous control). Muscle biopsies were taken before, immediately after, and 1, 2 and 4h after RE and feeding. Muscle protein synthesis (MPS) was measured by incorporation of [1, 2–13C2] leucine into myofibrillar proteins and the phosphorylation of p70S6K1 by immunoblotting. In young men, both area under the curve (AUC; FSR 0–4hP
Journal Article Type | Article |
---|---|
Acceptance Date | Apr 24, 2016 |
Online Publication Date | Apr 30, 2016 |
Publication Date | Jun 1, 2017 |
Deposit Date | May 5, 2016 |
Publicly Available Date | May 5, 2016 |
Journal | Clinical Nutrition |
Print ISSN | 0261-5614 |
Electronic ISSN | 1532-1983 |
Publisher | Elsevier |
Peer Reviewed | Peer Reviewed |
Volume | 36 |
Issue | 3 |
Pages | 888-895 |
DOI | https://doi.org/10.1016/j.clnu.2016.04.025 |
Keywords | Muscle; protein; nutrition; exercise; aging |
Public URL | https://nottingham-repository.worktribe.com/output/782953 |
Publisher URL | http://dx.doi.org/10.1016/j.clnu.2016.04.025 |
Additional Information | This article is maintained by: Elsevier; Article Title: Enriching a protein drink with leucine augments muscle protein synthesis after resistance exercise in young and older men; Journal Title: Clinical Nutrition; CrossRef DOI link to publisher maintained version: https://doi.org/10.1016/j.clnu.2016.04.025; Content Type: article; Copyright: © 2016 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism. All rights reserved. |
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Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by-nc-nd/4.0
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