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Maternal genome-wide DNA methylation profiling in gestational diabetes shows distinctive disease-associated changes relative to matched healthy pregnancies

Wu, Pensee; Farrell, William E.; Haworth, Kim E.; Emes, Richard D.; Kitchen, Mark O.; Glossop, John R.; Hanna, Fahmy W.; Fryer, Anthony A.

Authors

Pensee Wu

William E. Farrell

Kim E. Haworth

RICHARD EMES richard.emes@nottingham.ac.uk
Professor of Bioinformatics

Mark O. Kitchen

John R. Glossop

Fahmy W. Hanna

Anthony A. Fryer



Abstract

Several recent reports have described associations between gestational diabetes (GDM) and changes to the epigenomic landscape where the DNA samples were derived from either cord or placental sources. We employed genome-wide 450Karray analysis to determine changes to the epigenome in a unique cohort of maternal blood DNA from 11 pregnant women prior to GDM development relative to matched controls. Hierarchical clustering segregated the samples into two distinct clusters comprising GDM and healthy pregnancies. Screening identified 100 CpGs with a mean β-value difference of ≥0.2 between cases and controls. Using stringent criteria, 5 CpGs (within COPS8, PIK3R5, HAAO, CCDC124, and C5orf34 genes) demonstrated potentials to be clinical biomarkers as revealed by differential methylation in 8 of 11 women who developed GDM relative to matched controls. We identified, for the first time, maternal methylation changes prior to the onset of GDM that may prove useful as biomarkers for early therapeutic intervention.

Citation

Wu, P., Farrell, W. E., Haworth, K. E., Emes, R. D., Kitchen, M. O., Glossop, J. R., …Fryer, A. A. (2018). Maternal genome-wide DNA methylation profiling in gestational diabetes shows distinctive disease-associated changes relative to matched healthy pregnancies. Epigenetics, 13(2), 122-128. https://doi.org/10.1080/15592294.2016.1166321

Journal Article Type Article
Acceptance Date Mar 9, 2016
Online Publication Date Jan 25, 2018
Publication Date 2018-02
Deposit Date Oct 26, 2016
Publicly Available Date Jan 25, 2018
Journal Epigenetics
Print ISSN 1559-2294
Electronic ISSN 1559-2308
Publisher Taylor & Francis Open
Peer Reviewed Peer Reviewed
Volume 13
Issue 2
Pages 122-128
DOI https://doi.org/10.1080/15592294.2016.1166321
Keywords Cancer Research; Molecular Biology
Public URL http://eprints.nottingham.ac.uk/id/eprint/37929
Publisher URL http://www.tandfonline.com/doi/full/10.1080/15592294.2016.1166321
Copyright Statement Copyright information regarding this work can be found at the following address: http://eprints.nottingh.../end_user_agreement.pdf

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf





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