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Purification of a chymotrypsin-like enzyme present on adult Schistosoma mansoni worms from infected mice and its characterization as a host carboxylesterase

Igetei, Joseph E.; Liddell, Susan; El-Faham, Marwa; Doenhoff, Michael J.

Purification of a chymotrypsin-like enzyme present on adult Schistosoma mansoni worms from infected mice and its  characterization as a host carboxylesterase Thumbnail


Authors

Joseph E. Igetei

Susan Liddell

Marwa El-Faham

Michael J. Doenhoff



Abstract

A serine protease-like enzyme found in detergent extracts of Schistosoma mansoni adult worms perfused from infected mice has been purified from mouse blood and further characterized. The enzyme is approximately 85 kDa and hydrolyses N-acetyl-DL-phenylalanine β-naphthyl–ester, a chromogenic substrate for chymotrypsin-like enzymes. The enzyme from S. mansoni worms appears to be antigenically and enzymatically similar to a molecule that is present in normal mouse blood and so is seemingly host-derived. The enzyme was partially purified by depleting normal mouse serum of albumin using sodium chloride and cold ethanol, followed by repeated rounds of purification by one-dimensional sodium dodecyl sulphate polyacrylamide gel electrophoresis. The purified material was subjected to tandem mass spectrometry and its derived peptides found to belong to mouse carboxylesterase 1C. Its ability to hydrolyse α- or β-naphthyl acetates, which are general esterase substrates, has been confirmed. A similar carboxylesterase was purified and characterized from rat blood. Additional evidence to support identification of the enzyme as a carboxylesterase has been provided. Possible roles of the enzyme in the mouse host–parasite relationship could be to ease the passage of worms through the host's blood vessels and/or in immune evasion.

Citation

Igetei, J. E., Liddell, S., El-Faham, M., & Doenhoff, M. J. (2016). Purification of a chymotrypsin-like enzyme present on adult Schistosoma mansoni worms from infected mice and its characterization as a host carboxylesterase. Parasitology, 143(5), 646-657. https://doi.org/10.1017/S0031182016000184

Journal Article Type Article
Acceptance Date Jan 16, 2016
Online Publication Date Feb 29, 2016
Publication Date Apr 1, 2016
Deposit Date Feb 10, 2016
Publicly Available Date Feb 29, 2016
Journal Parasitology
Print ISSN 0031-1820
Electronic ISSN 1469-8161
Publisher Cambridge University Press
Peer Reviewed Peer Reviewed
Volume 143
Issue 5
Pages 646-657
DOI https://doi.org/10.1017/S0031182016000184
Keywords S. mansoni, host-derived enzyme, mice, carboxylesterase 1C, immune evasion
Public URL https://nottingham-repository.worktribe.com/output/777794
Publisher URL http://dx.doi.org/10.1017/S0031182016000184
Additional Information Copyright Cambridge University Press.
Contract Date Feb 10, 2016

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