Nicole Eisele
Placental expression of the angiogenic placental growth factor is stimulated by both aldosterone and simulated starvation
Eisele, Nicole; Albrecht, Christiane; Mistry, Hiten D.; Dick, Bernhard; Baumann, Marc; Surbeck, Daniel; Currie, Gemma; Delles, Christian; Mohaupt, Markus; Escher, Genevieve; Gennari-Moser, Carine
Authors
Christiane Albrecht
Hiten D. Mistry
Bernhard Dick
Marc Baumann
Daniel Surbeck
Gemma Currie
Christian Delles
Markus Mohaupt
Genevieve Escher
Carine Gennari-Moser
Abstract
Aldosterone is an important factor supporting placental growth and fetal development. Recently, expression of placental growth factor (PlGF) has been observed in response to aldosterone exposure in different models of atherosclerosis. Thus, we hypothesized that aldosterone up-regulates growth-adaptive angiogenesis in pregnancy, via increased placental PlGF expression.
We followed normotensive pregnant women (n = 24) throughout pregnancy and confirmed these results in a second independent first trimester cohort (n = 36). Urinary tetrahydroaldosterone was measured by gas chromatography-mass spectrometry and corrected for creatinine. Circulating PlGF concentrations were determined by ELISA. Additionally, cultured cell lines, adrenocortical H295R and choriocarcinoma BeWo cells, as well as primary human third trimester trophoblasts were tested in vitro. PlGF serum concentrations positively correlated with urinary tetrahydroaldosterone corrected for creatinine in these two independent cohorts. This observation was not due to PlGF, which did not induce aldosterone production in cultured H295R cells. On the other hand, PlGF expression was specifically enhanced by aldosterone in the presence of forskolin (p < 0.01) in trophoblasts. A pronounced stimulation of PlGF expression was observed with reduced glucose concentrations simulating starvation (p < 0.001).
In conclusion, aldosterone stimulates placental PlGF production, enhancing its availability during human pregnancy, a response amplified by reduced glucose supply. Given the crucial role of PlGF in maintaining a healthy pregnancy, these data support a key role of aldosterone for a healthy pregnancy outcome.
Citation
Eisele, N., Albrecht, C., Mistry, H. D., Dick, B., Baumann, M., Surbeck, D., Currie, G., Delles, C., Mohaupt, M., Escher, G., & Gennari-Moser, C. (2016). Placental expression of the angiogenic placental growth factor is stimulated by both aldosterone and simulated starvation. Placenta, 40, https://doi.org/10.1016/j.placenta.2016.02.004
Journal Article Type | Article |
---|---|
Acceptance Date | Feb 8, 2016 |
Online Publication Date | Feb 9, 2016 |
Publication Date | Apr 1, 2016 |
Deposit Date | Jul 21, 2017 |
Publicly Available Date | Jul 21, 2017 |
Journal | Placenta |
Print ISSN | 0143-4004 |
Electronic ISSN | 1532-3102 |
Publisher | Elsevier |
Peer Reviewed | Peer Reviewed |
Volume | 40 |
DOI | https://doi.org/10.1016/j.placenta.2016.02.004 |
Keywords | Aldosterone; Placental growth factor; Pregnancy; Glucose; Trophoblast |
Public URL | https://nottingham-repository.worktribe.com/output/777613 |
Publisher URL | http://www.sciencedirect.com/science/article/pii/S0143400416300248 |
Contract Date | Jul 21, 2017 |
Files
2016-02-01 Manuscript PlGF_Final rev HDM.pdf
(406 Kb)
PDF
Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by-nc-nd/4.0
Downloadable Citations
About Repository@Nottingham
Administrator e-mail: discovery-access-systems@nottingham.ac.uk
This application uses the following open-source libraries:
SheetJS Community Edition
Apache License Version 2.0 (http://www.apache.org/licenses/)
PDF.js
Apache License Version 2.0 (http://www.apache.org/licenses/)
Font Awesome
SIL OFL 1.1 (http://scripts.sil.org/OFL)
MIT License (http://opensource.org/licenses/mit-license.html)
CC BY 3.0 ( http://creativecommons.org/licenses/by/3.0/)
Powered by Worktribe © 2025
Advanced Search