Synthesis of triazole-linked morpholino oligonucleotides via Cu1 catalysed cycloaddition

Palframan, Matthew J.; Alharthy, Rima D.; Powalowska, Paulina K.; Hayes, Christopher J.

Abstract

Triazole-linked morpholino (TLMO) oligonucleic acids were synthesised using the CuI catalysed (3 + 2) azide–alkyne cycloaddition (CuAAC) reaction. The modified DNA analogues were incorporated into 13-mer sequences via solid phase synthesis. UV melting experiments showed that the TLMO modification gives higher Tm values than the corresponding TLDNA modification.

Citation

Palframan, M. J., Alharthy, R. D., Powalowska, P. K., & Hayes, C. J. (in press). Synthesis of triazole-linked morpholino oligonucleotides via Cu1 catalysed cycloaddition. Organic and Biomolecular Chemistry, 14, https://doi.org/10.1039/C6OB00007J

• Journal Article Type: Article
• Acceptance Date: Feb 16, 2016
• Online Publication Date: Feb 16, 2016
• Deposit Date: Jul 26, 2016
• Publicly Available Date: Jul 26, 2016
• Journal: Organic and Biomolecular Chemistry
• Print ISSN: 1477-0520
• Electronic ISSN: 1477-0520
• Publisher: Royal Society of Chemistry
• Peer Reviewed: Peer Reviewed
• Volume: 14
• DOI: https://doi.org/10.1039/C6OB00007J
• Public URL: https://nottingham-repository.worktribe.com/output/776187
• Publisher URL: http://dx.doi.org/10.1039/C6OB00007J