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Highly Anisotropic Suspended Planar-Array Chips with Multidimensional Sub-Micrometric Biomolecular Patterns

Agusil, Juan Pablo; Torras, N�ria; Duch, Marta; Esteve, Jaume; P�rez-Garc�a, Llu�sa; Samitier, Josep; Plaza, Jos� A.

Authors

Juan Pablo Agusil

N�ria Torras

Marta Duch

Jaume Esteve

Llu�sa P�rez-Garc�a

Josep Samitier

Jos� A. Plaza



Abstract

© 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Suspended planar-array (SPA) chips embody millions of individual miniaturized arrays to work in extremely small volumes. Here, the basis of a robust methodology for the fabrication of SPA silicon chips with on-demand physical and chemical anisotropies is demonstrated. Specifically, physical traits are defined during the fabrication process with special focus on the aspect ratio, branching, faceting, and size gradient of the final chips. Additionally, the chemical attributes augment the functionality of the chips with the inclusion of complete coverage or patterns of selected biomolecules on the surface of the chips with contact printing techniques, offering an extremely high versatility, not only with the choice of the pattern shape and distribution but also in the choice of biomolecular inks to pattern. This approach increases the miniaturization of printed arrays in 3D structures by two orders of magnitude compared to those previously demonstrated. Finally, functional micrometric and sub-micrometric patterned features are demonstrated with an antibody binding assay with the recognition of the printed spots with labeled antibodies from solution. The selective addition of physical and chemical attributes on the suspended chips represents the basis for future biomedical assays performed within extremely small volumes.

Journal Article Type Article
Acceptance Date Dec 21, 2016
Online Publication Date Feb 22, 2017
Publication Date Apr 5, 2017
Deposit Date Feb 5, 2018
Journal Advanced Functional Materials
Print ISSN 1616-301X
Electronic ISSN 1616-3028
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 27
Issue 13
Article Number 1605912
Pages 1-11
DOI https://doi.org/10.1002/adfm.201605912
Public URL https://nottingham-repository.worktribe.com/output/775981
Publisher URL https://onlinelibrary.wiley.com/doi/abs/10.1002/adfm.201605912


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