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Plasmodium P-type cyclin CYC3 modulates endomitotic growth during oocyst development in mosquitoes

Roques, Magali; Wall, Richard J; Douglass, Alexander P; Ramaprasad, Abhinay; Ferguson, David J P; Kaindama, Mbinda L; Brusini, Lorenzo; Joshi, Nimitray; Rchiad, Zineb; Brady, Declan; Guttery, David S; Wheatley, Sally P; Yamano, Hiroyuki; Holder, Anthony A; Pain, Arnab; Wickstead, Bill; Tewari, Rita

Authors

Magali Roques

Richard J Wall

Alexander P Douglass

Abhinay Ramaprasad

David J P Ferguson

Mbinda L Kaindama

Lorenzo Brusini

Nimitray Joshi

Zineb Rchiad

Declan Brady

David S Guttery

Hiroyuki Yamano

Anthony A Holder

Arnab Pain

RITA TEWARI RITA.TEWARI@NOTTINGHAM.AC.UK
Professor of Parasite Cell Biology



Abstract

Cell-cycle progression and cell division in eukaryotes are governed in part by the cyclin family and their regulation of cyclin-dependent kinases (CDKs). Cyclins are very well characterised in model systems such as yeast and human cells, but surprisingly little is known about their number and role in Plasmodium, the unicellular protozoan parasite that causes malaria. Malaria parasite cell division and proliferation differs from that of many eukaryotes. During its life cycle it undergoes two types of mitosis: endomitosis in asexual stages and an extremely rapid mitotic process during male gametogenesis. Both schizogony (producing merozoites) in host liver and red blood cells, and sporogony (producing sporozoites) in the mosquito vector, are endomitotic with repeated nuclear replication, without chromosome condensation, before cell division. The role of specific cyclins during Plasmodium cell proliferation was unknown. We show here that the Plasmodium genome contains only three cyclin genes, representing an unusual repertoire of cyclin classes. Expression and reverse genetic analyses of the single Plant (P)-type cyclin, CYC3, in the rodent malaria parasite, Plasmodium berghei, revealed a cytoplasmic and nuclear location of the GFP-tagged protein throughout the lifecycle. Deletion of cyc3 resulted in defects in size, number and growth of oocysts, with abnormalities in budding and sporozoite formation. Furthermore, global transcript analysis of the cyc3-deleted and wild type parasites at gametocyte and ookinete stages identified differentially expressed genes required for signalling, invasion and oocyst development. Collectively these data suggest that cyc3 modulates oocyst endomitotic development in Plasmodium berghei.

Citation

Roques, M., Wall, R. J., Douglass, A. P., Ramaprasad, A., Ferguson, D. J. P., Kaindama, M. L., …Tewari, R. (2015). Plasmodium P-type cyclin CYC3 modulates endomitotic growth during oocyst development in mosquitoes. PLoS Pathogens, 11(11), Article e1005273. https://doi.org/10.1371/journal.ppat.1005273

Journal Article Type Article
Acceptance Date Oct 21, 2015
Publication Date Nov 13, 2015
Deposit Date Jul 18, 2016
Publicly Available Date Jul 18, 2016
Journal PloS Pathogens
Print ISSN 1553-7366
Electronic ISSN 1553-7374
Publisher Public Library of Science
Peer Reviewed Peer Reviewed
Volume 11
Issue 11
Article Number e1005273
DOI https://doi.org/10.1371/journal.ppat.1005273
Public URL https://nottingham-repository.worktribe.com/output/766937
Publisher URL http://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1005273

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0





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