Imprinted contact lenses for sustained release of polymyxin B and related antimicrobial peptides

Malakooti, Negin; Alexander, Cameron; Alvarez-Lorenzo, Carmen

Abstract

The aim of this work was to develop drug-soft contact lens combination products suitable for controlled release of antimicrobial peptides on the ocular surface. Incorporation of functional monomers and the application of molecular imprinting techniques were explored to endow 2-hydroxyethyl methacrylate (HEMA) hydrogels with the ability to load and to sustain the release of polymyxin B and vancomycin. Various HEMA–drug–functional monomer–cross–linker molar ratios were evaluated to prepare polymyxin B imprinted and non-imprinted hydrogels. Acrylic acid-functionalized and imprinted hydrogels loaded greater amounts of polymyxin B and led to more sustained release profiles, in comparison with non-functionalized and non-imprinted networks. Polymyxin B-loaded hydrogels showed good biocompatibility in hen’s egg test-chorioallantoic membrane tests. Functionalized hydrogels also loaded vancomycin and sustained its release, but the imprinting effect was only exhibited with polymyxin B, as demonstrated in rebinding tests. Microbiological assays carried out with Pseudomonas aeruginosa allowed identification of the most suitable hydrogel composition for efficient bacteria eradication; some hydrogels being able to stand several continued challenges against this important bacterial pathogen.

Citation

Malakooti, N., Alexander, C., & Alvarez-Lorenzo, C. (2015). Imprinted contact lenses for sustained release of polymyxin B and related antimicrobial peptides. Journal of Pharmaceutical Sciences, 104(10), https://doi.org/10.1002/jps.24537

• Journal Article Type: Article
• Acceptance Date: May 15, 2015
• Online Publication Date: Jun 10, 2015
• Publication Date: Oct 31, 2015
• Deposit Date: Nov 30, 2016
• Publicly Available Date: Nov 30, 2016
• Journal: Journal of Pharmaceutical Sciences
• Print ISSN: 0022-3549
• Electronic ISSN: 1520-6017
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 104
• Issue: 10
• DOI: https://doi.org/10.1002/jps.24537
• Keywords: Hydrogels; Biomaterials; Controlled release; Peptide delivery; Mucosal delivery; Drug-device combination product; Molecular imprinting; Ophthalmic drug delivery; Contact lens; Pseudomonas aeruginosa
• Public URL: https://nottingham-repository.worktribe.com/output/762800
• Publisher URL: http://dx.doi.org/10.1002/jps.24537
• Contract Date: Nov 30, 2016