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Hop proanthocyanidins for the fining of beer

Linforth, Rob S.T.; Westwood, K.; Somani, Abhishek; Cook, David J.

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Authors

Rob S.T. Linforth

K. Westwood

Abhishek Somani

Profile image of DAVID COOK

DAVID COOK david.cook@nottingham.ac.uk
Sabmiller Chair Brewing Science



Abstract

Fining agents are used in the clarification of beers; they help to reduce the time required to sediment suspended yeast cells and ensure the clarity and colloidal stability of beer. Following an adventitious observation during dry-hopping experiments, we identified a fining activity associated with Saaz hops. Extracts of hop cones were subsequently shown to have the capacity to flocculate yeast and result in their sedimentation. This activity has since been identified in extracts of many different hop varieties and, significantly in spent hops, the co-product resulting from commercial extraction of hops with either CO2 or ethanol. Here we illustrate the activity of the novel finings extracted from spent hops following CO2 extraction of Galena hops. The sediments formed on fining were compact, relative to those obtained when commercial isinglass was used to fine the same beers. The hop extracts were also effective in reducing 90° haze in beers under conditions designed to mimic both cask ale (12 °C) and lager (4 °C) type applications. The compounds responsible for the fining activity appear to be large (30–100 kDa, or more) polyphenols. Analysis of the polyphenols using colourimetric tests indicated the presence of proanthocyanidins. On acidic hydrolysis these generated cyanidin, which would be derived from a polymer composed of catechin and epicatechin subunits. The presence of these materials in spent hops offers the possibility to develop commercial products, with desirable fining properties, from an existing co-product stream. Furthermore, the finings are derived from a traditional ingredient of the brewing process.

Citation

Linforth, R. S., Westwood, K., Somani, A., & Cook, D. J. (in press). Hop proanthocyanidins for the fining of beer. Journal of the Institute of Brewing, 121(4), https://doi.org/10.1002/jib.250

Journal Article Type Article
Acceptance Date Jul 11, 2015
Online Publication Date Sep 16, 2015
Deposit Date Sep 18, 2017
Publicly Available Date Sep 18, 2017
Journal Journal of the Insitute of Brewing
Electronic ISSN 2050-0416
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 121
Issue 4
DOI https://doi.org/10.1002/jib.250
Keywords fining agent; hop polyphenolics; proanthocyanidin; brewing; colloidal stability
Public URL https://nottingham-repository.worktribe.com/output/761070
Publisher URL http://onlinelibrary.wiley.com/doi/10.1002/jib.250/abstract
Additional Information This is the peer reviewed version of the following article: Linforth, R. S. T., Westwood, K., Somani, A., Doherty, N., and Cook, D. J. (2015) Hop proanthocyanidins for the fining of beer. Journal of the Institute of Brewing, 121: 490–495., which has been published in final form at http://dx.doi.org/10.1002/jib.250. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
Contract Date Sep 18, 2017

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