Skip to main content

Research Repository

Advanced Search

Characterization of multinucleated giant cells in synovium and subchondral bone in knee osteoarthritis and rheumatoid arthritis

Prieto-Potin, Iván; Largo, Raquel; Roman-Blas, Jorge A.; Herrero-Beaumont, Gabriel; Walsh, David A.

Authors

Iván Prieto-Potin

Raquel Largo

Jorge A. Roman-Blas

Gabriel Herrero-Beaumont

David A. Walsh



Abstract

Background: Multinucleated giant cells have been noticed in diverse arthritic conditions since their first description in rheumatoid synovium. However, their role in the pathogenesis of osteoarthritis (OA) or rheumatoid arthritis (RA) still remains broadly unknown. We aimed to study the presence and characteristics of multinucleated giant cells (MGC) both in synovium and in subchondral bone tissues of patients with OA or RA.
Methods: Knee synovial and subchondral bone samples were from age-matched patients undergoing total joint replacement for OA or RA, or non-arthritic post mortem (PM) controls. OA synovium was stratified by histological inflammation grade using index tissue sections. Synovitis was assessed by Krenn score. Histological studies employed specific antibodies against macrophage markers or cathepsin K, or TRAP enzymatic assay.
Results: Inflamed OA and RA synovia displayed more multinucleated giant cells than did non-inflamed OA and PM synovia. There was a significant association between MGC numbers and synovitis severity. A TRAP negative/cathepsin K negative Langhans-like subtype was predominant in OA, whereas both Langhans-like and TRAP-positive/ cathepsin K negative foreign-body-like subtypes were most commonly detected in RA. Plasma-like and foam-like subtypes also were observed in OA and RA synovia, and the latter was found surrounding adipocytes. TRAP positive/ cathepsin K positive osteoclasts were only identified adjacent to subchondral bone surfaces. TRAP positive osteoclasts were significantly increased in subchondral bone in OA and RA compared to PM controls.
Conclusions: Multinucleated giant cells are associated with synovitis severity, and subchondral osteoclast numbers are increased in OA, as well as in RA. Further research targeting multinucleated giant cells is warranted to elucidate their contributions to the symptoms and joint damage associated with arthritis.

Journal Article Type Article
Publication Date Aug 27, 2015
Journal BMC Musculoskeletal Disorders
Electronic ISSN 1471-2474
Publisher Springer Verlag
Peer Reviewed Peer Reviewed
Volume 16
Article Number 226
APA6 Citation Prieto-Potin, I., Largo, R., Roman-Blas, J. A., Herrero-Beaumont, G., & Walsh, D. A. (2015). Characterization of multinucleated giant cells in synovium and subchondral bone in knee osteoarthritis and rheumatoid arthritis. BMC Musculoskeletal Disorders, 16, https://doi.org/10.1186/s12891-015-0664-5
DOI https://doi.org/10.1186/s12891-015-0664-5
Keywords Rheumatoid Arthritis; Synovitis; Subchondral Bone; Multinucleated Giant Cell; Rheumatoid Arthritis Synovia
Publisher URL https://bmcmusculoskeletdisord.biomedcentral.com/articles/10.1186/s12891-015-0664-5
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0

Files

Prieto_Potin et al_characterisation of multimucleated gian cells.pdf (1.6 Mb)
PDF

Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0





You might also like



Downloadable Citations

;