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Direct RT-qPCR Assay for the Detection of SARS-CoV-2 in Saliva Samples

Tarantini, Francesco Saverio; Wu, Siyu; Jenkins, Harry; Lopez, Ana Tellechea; Tomlin, Hannah; Hyde, Ralph; Lis-Slimak, Katarzyna; Thompson, Jamie Louise; Pijuan-Galitó, Sara; Scales, Danielle; Kaneko, Kazuyo; Dey, Jayasree; Park, Emily; Hill, Jack; Lee, I. Ning; Doolan, Lara; Arendt-Tranholm, Asta; Denning, Chris; Seedhouse, Claire; Benest, Andrew V.

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Authors

Siyu Wu

Harry Jenkins

Ana Tellechea Lopez

Hannah Tomlin

Ralph Hyde

Katarzyna Lis-Slimak

Jamie Louise Thompson

Sara Pijuan-Galitó

Danielle Scales

Kazuyo Kaneko

Jayasree Dey

Emily Park

Jack Hill

I-NING LEE I-NING.LEE@NOTTINGHAM.AC.UK
Research Fellow

Lara Doolan

Asta Arendt-Tranholm

CHRIS DENNING chris.denning@nottingham.ac.uk
Professor of Stem Cell Biology



Abstract

Since mid-2020 there have been complexities and difficulties in the standardisation and administration of nasopharyngeal swabs. Coupled with the variable and/or poor accuracy of lateral flow devices, this has led to increased societal ‘testing fatigue’ and reduced confidence in test results. Consequently, asymptomatic individuals have developed reluctance towards repeat testing, which remains the best way to monitor COVID-19 cases in the wider population. On the other hand, saliva-based PCR, a non-invasive, highly sensitive, and accurate test suitable for everyone, is gaining momentum as a straightforward and reliable means of detecting SARS-CoV-2 in symptomatic and asymptomatic individuals. Here, we provide an itemised list of the equipment and reagents involved in the process of sample submission, inactivation and analysis, as well as a detailed description of how each of these steps is performed.

Journal Article Type Article
Acceptance Date Mar 2, 2022
Online Publication Date Mar 7, 2022
Publication Date Mar 7, 2022
Deposit Date Mar 25, 2022
Publicly Available Date Mar 25, 2022
Journal Methods and Protocols
Electronic ISSN 2409-9279
Publisher MDPI AG
Peer Reviewed Peer Reviewed
Volume 5
Issue 2
Article Number 25
DOI https://doi.org/10.3390/mps5020025
Keywords Biochemistry, Genetics and Molecular Biology (miscellaneous); Structural Biology; Biotechnology
Public URL https://nottingham-repository.worktribe.com/output/7578099
Publisher URL https://www.mdpi.com/2409-9279/5/2/25

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