Sinead E. Shortall
Contribution of serotonin and dopamine to changes in core body temperature and locomotor activity in rats following repeated administration of mephedrone
Shortall, Sinead E.; Spicer, Clare H.; Ebling, Francis J.P.; Green, A. Richard; Fone, Kevin C.F.; King, Madeleine V.
Authors
Clare H. Spicer
Francis J.P. Ebling
A. Richard Green
Kevin C.F. Fone
MADELEINE KING madeleine.king@nottingham.ac.uk
Assistant Professor
Abstract
© 2015 Society for the Study of Addiction The psychoactive effects of mephedrone are commonly compared with those of 3,4-methylenedioxymethamphetamine, but because of a shorter duration of action, users often employ repeated administration to maintain its psychoactive effects. This study examined the effects of repeated mephedrone administration on locomotor activity, body temperature and striatal dopamine and 5-hydroxytryptamine (5-HT) levels and the role of dopaminergic and serotonergic neurons in these responses. Adult male Lister hooded rats received three injections of vehicle (1 ml/kg, i.p.) or mephedrone HCl (10 mg/kg) at 2 h intervals for radiotelemetry (temperature and activity) or microdialysis (dopamine and 5-HT) measurements. Intracerebroventricular pre-treatment (21 to 28 days earlier) with 5,7-dihydroxytryptamine (150 µg) or 6-hydroxydopamine (300 µg) was used to examine the impact of 5-HT or dopamine depletion on mephedrone-induced changes in temperature and activity. A final study examined the influence of i.p. pre-treatment (−30 min) with the 5-HT 1A receptor antagonist WAY-100635 (0.5 mg/kg), 5-HT 1B receptor antagonist GR 127935 (3 mg/kg) or the 5-HT 7 receptor antagonist SB-258719 (10 mg/kg) on mephedrone-induced changes in locomotor activity and rectal temperature. Mephedrone caused rapid-onset hyperactivity, hypothermia (attenuated on repeat dosing) and increased striatal dopamine and 5-HT release following each injection. Mephedrone-induced hyperactivity was attenuated by 5-HT depletion and 5-HT 1B receptor antagonism, whereas the hypothermia was completely abolished by 5-HT depletion and lessened by 5-HT 1A receptor antagonism. These findings suggest that stimulation of central 5-HT release and/or inhibition of 5-HT reuptake play a pivotal role in both the hyperlocomotor and hypothermic effects of mephedrone, which are mediated in part via 5-HT 1B and 5-HT 1A receptors.
Citation
Shortall, S. E., Spicer, C. H., Ebling, F. J., Green, A. R., Fone, K. C., & King, M. V. (2016). Contribution of serotonin and dopamine to changes in core body temperature and locomotor activity in rats following repeated administration of mephedrone. Addiction Biology, 21(6), 1127-1139. https://doi.org/10.1111/adb.12283
Journal Article Type | Article |
---|---|
Acceptance Date | Jun 12, 2015 |
Online Publication Date | Jul 16, 2015 |
Publication Date | Nov 1, 2016 |
Deposit Date | Sep 21, 2015 |
Publicly Available Date | Sep 21, 2015 |
Journal | Addiction Biology |
Print ISSN | 1355-6215 |
Electronic ISSN | 1369-1600 |
Publisher | Wiley |
Peer Reviewed | Peer Reviewed |
Volume | 21 |
Issue | 6 |
Pages | 1127-1139 |
DOI | https://doi.org/10.1111/adb.12283 |
Keywords | 5-HT, dopamine, locomotor activity, mephedrone, microdialysis, telemetry |
Public URL | https://nottingham-repository.worktribe.com/output/756816 |
Publisher URL | http://onlinelibrary.wiley.com/doi/10.1111/adb.12283/abstract |
Contract Date | Sep 21, 2015 |
Files
Shortall 2015 AddictionBio.pdf
(377 Kb)
PDF
You might also like
Neural correlates of hyperalgesia in the monosodium iodoacetate model of osteoarthritis pain
(2016)
Journal Article
Early life programming of fear conditioning and extinction in adult male rats
(2009)
Journal Article
Early life programming of innate fear and fear learning in adult female rats
(2008)
Journal Article
Neonatal maternal separation alters reward-related ultrasonic vocalizations in rat dams
(2009)
Journal Article
Downloadable Citations
About Repository@Nottingham
Administrator e-mail: discovery-access-systems@nottingham.ac.uk
This application uses the following open-source libraries:
SheetJS Community Edition
Apache License Version 2.0 (http://www.apache.org/licenses/)
PDF.js
Apache License Version 2.0 (http://www.apache.org/licenses/)
Font Awesome
SIL OFL 1.1 (http://scripts.sil.org/OFL)
MIT License (http://opensource.org/licenses/mit-license.html)
CC BY 3.0 ( http://creativecommons.org/licenses/by/3.0/)
Powered by Worktribe © 2024
Advanced Search