Assessment of caecal parameters in layer hens fed on diets containing wheat distillers dried grains with solubles

White, Gavin A.; Richards, P. J.; Wu, S.; Mellits, K. H.; Wiseman, J.

Abstract

There is much interest in quantifying the nutritional value of UK wheat distillers dried grains with solubles (W-DDGS) for livestock species. A study was designed to evaluate caecal parameters (pH, short chain fatty acids (SCFAs) and bacterial diversity) in layer hens fed on balanced diets containing graded concentrations of W-DDGS.
A total of 32 layer hens (Bovans Brown strain at 27 weeks of age) were randomly allocated to one of 4 dietary treatments containing W-DDGS at 0, 60, 120 or 180 g/kg. Each treatment was fed to 8 replicate individually housed layer hens over a 5-d acclimatisation period, followed by a 4-week trial. Individual feed intakes were monitored and all eggs were collected daily for weeks 2, 3 and 4 of the trial, weighed and an assessment of eggshell “dirtiness” made. All hens were culled on d 29 and caecal pH and SCFAs measured. Polymerase chain reaction denaturing gradient gel electrophoresis of the bacterial 16 S rDNA gene was used to assess total bacterial diversity of luminal caecal content from hens fed the 0 and 180 g W-DDGS/kg diets. Unweighted pair group method with arithmetic mean (UPGMA) dendrograms were generated from DGGE banding patterns.
Increasing W-DDGS dietary concentrations resulted in a more acidic caecal environment. Caecal SCFAs were unaffected by diet aside from a quadratic effect for molar proportions of isobutyric acid. Diversity profiles of the bacterial 16S rRNA gene from luminal caecal contents were unaffected by W-DDGS inclusion.
The results of the current study suggest that W-DDGS can be successfully formulated into nutritionally balanced layer diets (supplemented with xylanase and phytase) at up to 180 g/kg with no detrimental effects to the caecal environment.

Citation

White, G. A., Richards, P. J., Wu, S., Mellits, K. H., & Wiseman, J. (2015). Assessment of caecal parameters in layer hens fed on diets containing wheat distillers dried grains with solubles. British Poultry Science, 56(4), 494-502. https://doi.org/10.1080/00071668.2015.1064859

• Journal Article Type: Article
• Acceptance Date: May 14, 2015
• Online Publication Date: Aug 3, 2015
• Publication Date: Jul 4, 2015
• Deposit Date: Dec 8, 2016
• Publicly Available Date: Dec 8, 2016
• Journal: British Poultry Science
• Print ISSN: 0007-1668
• Electronic ISSN: 1466-1799
• Publisher: Taylor and Francis
• Peer Reviewed: Peer Reviewed
• Volume: 56
• Issue: 4
• Pages: 494-502
• DOI: https://doi.org/10.1080/00071668.2015.1064859
• Public URL: https://nottingham-repository.worktribe.com/output/754038
• Publisher URL: http://www.tandfonline.com/doi/full/10.1080/00071668.2015.1064859
• Additional Information: Peer Review Statement: The publishing and review policy for this title is described in its Aims & Scope.; Aim & Scope: http://www.tandfonline.com/action/journalInformation?show=aimsScope&journalCode=cbps20
• Contract Date: Dec 8, 2016