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Dual signal transduction pathways activated by TSH receptors in rat primary tanycyte cultures

Bolborea, Matei; Helfer, Gisela; Ebling, Francis J.P.; Barrett, Perry

Authors

Matei Bolborea

Gisela Helfer

Francis J.P. Ebling fran.ebling@nottingham.ac.uk

Perry Barrett



Abstract

Tanycytes play multiple roles in hypothalamic functions, including sensing peripheral nutrients and metabolic hormones, regulating neurosecretion and mediating seasonal cycles of reproduction and metabolic physiology. This last function reflects the expression of TSH receptors in tanycytes, which detect photoperiod-regulated changes in TSH secretion from the neighbouring pars tuberalis. The present overall aim was to determine the signal transduction pathway by which TSH signals in tanycytes. Expression of the TSH receptor in tanycytes of 10-day-old Sprague Dawley rats was observed by in situ hybridisation. Primary ependymal cell cultures prepared from 10-day-old rats were found by immunohistochemistry to express vimentin but not GFAP and by PCR to express mRNA for Dio2, Gpr50, Darpp-32 and Tsh receptors that are characteristic of tanycytes. Treatment of primary tanycyte/ependymal cultures with TSH (100 IU/l) increased cAMP as assessed by ELISA and induced a cAMP-independent increase in the phosphorylation of ERK1/2 as assessed by western blot analysis. Furthermore, TSH (100 IU/l) stimulated a 2.17-fold increase in Dio2 mRNA expression. We conclude that TSH signal transduction in cultured tanycytes signals via Gαs to increase cAMP and via an alternative G protein to increase phosphorylation of ERK1/2.

Journal Article Type Article
Publication Date Jun 1, 2015
Journal Journal of Molecular Endocrinology
Print ISSN 0952-5041
Electronic ISSN 1479-6813
Publisher BioScientifica
Peer Reviewed Peer Reviewed
Volume 54
APA6 Citation Bolborea, M., Helfer, G., Ebling, F. J., & Barrett, P. (2015). Dual signal transduction pathways activated by TSH receptors in rat primary tanycyte cultures. Journal of Molecular Endocrinology, 54, https://doi.org/10.1530/JME-14-0298
DOI https://doi.org/10.1530/JME-14-0298
Keywords Thyrotrophin, Pars tuberalis, Median eminence, Neuroendocrine
Publisher URL http://jme.endocrinology-journals.org/content/54/3/241
Copyright Statement Copyright information regarding this work can be found at the following address: http://eprints.nottingh.../end_user_agreement.pdf
Additional Information Disclaimer: this is not the definitive version of record of this article. This manuscript has been accepted for publication in Journal of Molecular Endocrinology, but the version presented here has not yet been copy-edited, formatted or proofed. Consequently, Bioscientifica accepts no responsibility for any errors or omissions it may contain. The definitive version is now freely available at 10.1530/JME-14-0298 2015.

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf





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