Skip to main content

Research Repository

Advanced Search

The contribution of PA-X to the virulence of pandemic 2009 H1N1 and highly pathogenic H5N1 avian influenza viruses

Gao, Huijie; Sun, Yipeng; Hu, Jiao; Qi, Lu; Wang, Jinliang; Xiong, Xin; Wang, Yu; He, Qiming; Lin, Yang; Kong, Weili; Seng, Lai-Giea; Sun, Honglei; Pu, Juan; Chang, Kin-Chow; Liu, Xiufan; Liu, Jinhua

Authors

Huijie Gao

Yipeng Sun

Jiao Hu

Lu Qi

Jinliang Wang

Xin Xiong

Yu Wang

Qiming He

Yang Lin

Weili Kong

Lai-Giea Seng

Honglei Sun

Juan Pu

KIN-CHOW CHANG kin-chow.chang@nottingham.ac.uk
Professor of Veterinary Molecular Medicine

Xiufan Liu

Jinhua Liu



Abstract

PA-X is a novel protein encoded by PA mRNA and is found to decrease the pathogenicity of pandemic 1918 H1N1 virus in mice. However, the importance of PA-X proteins in current epidemiologically important influenza A virus strains is not known. In this study, we report on the pathogenicity and pathological effects of PA-X deficient 2009 pandemic H1N1 (pH1N1) and highly pathogenic avian influenza H5N1 viruses. We found that loss of PA-X expression in pH1N1 and H5N1 viruses increased viral replication and apoptosis in A549 cells and increased virulence and host inflammatory response in mice. In addition, PA-X deficient pH1N1 and H5N1 viruses up-regulated PA mRNA and protein synthesis and increased viral polymerase activity. Loss of PA-X was also accompanied by accelerated nuclear accumulation of PA protein and reduced suppression of PA on non-viral protein expression. Our study highlights the effects of PA-X on the moderation of viral pathogenesis and pathogenicity.

Citation

Gao, H., Sun, Y., Hu, J., Qi, L., Wang, J., Xiong, X., …Liu, J. (in press). The contribution of PA-X to the virulence of pandemic 2009 H1N1 and highly pathogenic H5N1 avian influenza viruses. Scientific Reports, 5(1), https://doi.org/10.1038/srep08262

Journal Article Type Article
Acceptance Date Jan 14, 2015
Online Publication Date Feb 5, 2015
Deposit Date Jul 5, 2017
Publicly Available Date Jul 5, 2017
Journal Scientific Reports
Print ISSN 2045-2322
Electronic ISSN 2045-2322
Publisher Nature Publishing Group
Peer Reviewed Peer Reviewed
Volume 5
Issue 1
DOI https://doi.org/10.1038/srep08262
Public URL http://eprints.nottingham.ac.uk/id/eprint/43991
Publisher URL https://www.nature.com/articles/srep08262.pdf
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0

Files

srep08262.pdf (2.1 Mb)
PDF

Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0





You might also like



Downloadable Citations