Quantum dot labelling of adenovirus allows for highly sensitive single cell flow and imaging cytometry

Herod, Morgan R.; Pineda, Robert G.; Mautner, Vivien; Onion, David

Abstract

A quantum dot method for highly efficient labelling of single adenoviral particles is developed. The technique has no impact on viral fitness and allows the imaging and tracking of virus binding and internalisation events using a variety of techniques including imaging cytometry and confocal microscopy. The method is applied to characterise the tropism of different adenoviral vectors.

Citation

Herod, M. R., Pineda, R. G., Mautner, V., & Onion, D. (2015). Quantum dot labelling of adenovirus allows for highly sensitive single cell flow and imaging cytometry. Small, 11(7), https://doi.org/10.1002/smll.201401885

• Journal Article Type: Article
• Publication Date: Feb 18, 2015
• Deposit Date: Jan 20, 2016
• Publicly Available Date: Jan 20, 2016
• Journal: Small
• Print ISSN: 1613-6810
• Electronic ISSN: 1613-6829
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 11
• Issue: 7
• DOI: https://doi.org/10.1002/smll.201401885
• Keywords: quantum dots; adenovirus; gene therapy; flow cytometry; imaging cytometry
• Public URL: https://nottingham-repository.worktribe.com/output/744811
• Publisher URL: http://onlinelibrary.wiley.com/doi/10.1002/smll.201401885/full