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Quantum dot labelling of adenovirus allows for highly sensitive single cell flow and imaging cytometry

Herod, Morgan R.; Pineda, Robert G.; Mautner, Vivien; Onion, David

Authors

Robert G. Pineda

Vivien Mautner

David Onion david.onion@nottingham.ac.uk



Abstract

A quantum dot method for highly efficient labelling of single adenoviral particles is developed. The technique has no impact on viral fitness and allows the imaging and tracking of virus binding and internalisation events using a variety of techniques including imaging cytometry and confocal microscopy. The method is applied to characterise the tropism of different adenoviral vectors.

Journal Article Type Article
Publication Date Feb 18, 2015
Journal Small
Print ISSN 1613-6810
Electronic ISSN 1613-6829
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 11
Issue 7
APA6 Citation Herod, M. R., Pineda, R. G., Mautner, V., & Onion, D. (2015). Quantum dot labelling of adenovirus allows for highly sensitive single cell flow and imaging cytometry. Small, 11(7), doi:10.1002/smll.201401885
DOI https://doi.org/10.1002/smll.201401885
Keywords quantum dots; adenovirus; gene therapy; flow cytometry; imaging cytometry
Publisher URL http://onlinelibrary.wiley.com/doi/10.1002/smll.201401885/full
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0





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