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Precision assembly of complex cellular microenvironments using holographic optical tweezers

Kirkham, Glen R.; Britchford, Emily; Upton, Thomas; Ware, James; Gibson, Graham; Devaud, Yannick; Ehrbar, Martin; Padgett, Miles; Allen, Stephanie; Buttery, Lee D.K.; Shakesheff, Kevin M.

Authors

Glen R. Kirkham

Emily Britchford

Thomas Upton

James Ware jamiew4430@gmail.com

Graham Gibson

Yannick Devaud

Martin Ehrbar

Miles Padgett

STEPHANIE ALLEN stephanie.allen@nottingham.ac.uk
Professor of Pharmaceutical Biophysics

Lee D.K. Buttery



Abstract

The accurate study of cellular microenvironments is limited by the lack of technologies that can manipulate cells in 3D at a sufficiently small length scale. The ability to build and manipulate multicellular microscopic structures will facilitate a more detailed understanding of cellular function in fields such as developmental and stem cell biology. We present a holographic optical tweezers based technology to accurately generate bespoke cellular micro-architectures. Using embryonic stem cells, 3D structures of varying geometries were created and stabilized using hydrogels and cell-cell adhesion methods. Control of chemical microenvironments was achieved by the temporal release of specific factors from polymer microparticles positioned within these constructs. Complex co-culture micro-environmental analogues were also generated to reproduce structures found within adult stem cell niches. The application of holographic optical tweezers-based micromanipulation will enable novel insights into biological microenvironments by allowing researchers to form complex architectures with sub-micron precision of cells, matrices and molecules.

Journal Article Type Article
Publication Date Feb 26, 2015
Journal Scientific Reports
Print ISSN 2045-2322
Electronic ISSN 2045-2322
Publisher Nature Publishing Group
Peer Reviewed Peer Reviewed
Volume 5
Article Number 8577
APA6 Citation Kirkham, G. R., Britchford, E., Upton, T., Ware, J., Gibson, G., Devaud, Y., …Shakesheff, K. M. (2015). Precision assembly of complex cellular microenvironments using holographic optical tweezers. Scientific Reports, 5, https://doi.org/10.1038/srep08577
DOI https://doi.org/10.1038/srep08577
Publisher URL https://www.nature.com/articles/srep08577
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0





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