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3D chemical characterization of frozen hydrated hydrogels using ToF-SIMS with argon cluster sputter depth profiling

Taylor, Michael; Scurr, David; Lutolf, Matthias; Buttery, Lee D.K.; Zelzer, Mischa; Alexander, Morgan R.

Authors

Michael Taylor

DAVID SCURR david.scurr@nottingham.ac.uk
Senior Research Fellow in Surface Analysis

Matthias Lutolf

Lee D.K. Buttery



Abstract

Hydrogels have been used extensively in bioengineering as artificial cell culture supports. Investigation of the interrelationship between cellular response to the hydrogel and its chemistry ideally requires methods that allow characterization without labels and can map species in three dimensional to follow biomolecules adsorbed to, and absorbed into, the open structure before and during culture. Time-of-flight secondary ion mass spectrometry (ToF-SIMS) has the potential to be utilized for through thickness characterization of hydrogels. The authors have established a simple sample preparation procedure to successfully achieve analysis of frozen hydrated hydrogels using ToF-SIMS without the need for dry glove box entry equipment. They demonstrate this on a poly(2-hydroxyethyl methacrylate) (pHEMA) film where a model protein (lysozyme) is incorporated using two methods to demonstrate how protein distribution can be determined. A comparison of lysozyme incorporation is made between the situation where the protein is present in a polymer dip coating solution and where lysozyme is in an aqueous medium in which the film is incubated. It is shown that protonated water clusters H(H2O)nþ where n ¼ 5–11 that are indicative of ice are detected through the entire thickness of the pHEMA. The lysozyme distribution through the pHEMA hydrogel films can be determined using the intensity of a characteristic amino acid secondary ion fragment.

Journal Article Type Article
Publication Date Aug 7, 2015
Journal Biointerphases
Print ISSN 1934-8630
Electronic ISSN 1934-8630
Publisher AIP Publishing
Peer Reviewed Peer Reviewed
Volume 11
Issue 2
Article Number 02A301
APA6 Citation Taylor, M., Scurr, D., Lutolf, M., Buttery, L. D., Zelzer, M., & Alexander, M. R. (2015). 3D chemical characterization of frozen hydrated hydrogels using ToF-SIMS with argon cluster sputter depth profiling. Biointerphases, 11(2), https://doi.org/10.1116/1.4928209
DOI https://doi.org/10.1116/1.4928209
Keywords Gels, Ice, Cell Cultures, Polymer films, Polymers
Publisher URL http://scitation.aip.org/content/avs/journal/bip/11/2/10.1116/1.4928209
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0
Additional Information The publications listed below are published by the American Vacuum Society. © 2015 American Vacuum Society. All rights reserved. No claim is made to original U.S. Government works.

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0





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