Casper P. Hagen
Longitudinal assessment of circulating insulin-like peptide 3 levels in healthy peripubertal girls
Hagen, Casper P.; Mieritz, Mikkel G.; Nielsen, John E.; Anand-Ivell, Ravinder; Ivell, Richard; Juul, Anders
Mikkel G. Mieritz
John E. Nielsen
RAVINDER ANAND-IVELL Ravinder.Anandfirstname.lastname@example.org
Richard Ivell email@example.com
Objective: To elucidate the natural course of circulating insulin-like peptide 3 (INSL3) levels according to puberty as well as its relation to other reproductive hormones.
Design: Population-based cohort study.
Setting: Not applicable.
Patient(s): Healthy peripubertal girls (n¼10) examined every 6 months; total number of examinations was 84; median (range) per girl:9 (4–10), including staging of pubertal breast development and blood samples.
Main Outcome Measure(s): Serum levels of INSL3, inhibin B, E2, antim€ullerian hormone, LH, and FSH (validated immunoassays), and T and androstenedione (liquid chromatography–tandem mass spectrometry).
Result(s): Serum levels of INSL3 varied considerably between girls (range, 0.01–0.27 ng/mL) and within each girl as puberty progressed; intraindividual variation, median (range) 102% (65%–143%). Insulin-like peptide 3 increased in late puberty (B1 to B4þB5); geometric mean 0.03 ng/mL to 0.15 ng/mL. Insulin-like peptide 3 levels reflected markers of large follicles (T, androstendione, inhibin B, and E2) better than markers of small follicles (antim€ullerian hormone), and INSL3 staining was localized in theca interna cells of antral follicles.
Conclusion(s): Insulin-like peptide 3 increased in late puberty, albeit inter- and intraindividual variations were substantial. Immunohistochemistry and intraindividual variation, as well as relations to other ovarian hormones, reveal that INSL3 in girls is a unique and specific marker of theca cells surrounding antral follicles. The potential clinical use of INSL3 for evaluation of ovarian function in girls remains to be elucidated.
|Journal Article Type||Article|
|Publication Date||Mar 1, 2015|
|Journal||Fertility and Sterility|
|Peer Reviewed||Peer Reviewed|
|APA6 Citation||Hagen, C. P., Mieritz, M. G., Nielsen, J. E., Anand-Ivell, R., Ivell, R., & Juul, A. (2015). Longitudinal assessment of circulating insulin-like peptide 3 levels in healthy peripubertal girls. Fertility and Sterility, 103(3), doi:10.1016/j.fertnstert.2014.11.014|
|Keywords||INSL3, puberty, theca, girl, follicle|
|Copyright Statement||Copyright information regarding this work can be found at the following address: http://eprints.nottingh.../end_user_agreement.pdf|
You might also like
Effects of acute hCG stimulation on serum INSL3 and 25‐OH vitamin D in Klinefelter syndrome
Prepubertal nutrition alters Leydig cell functional capacity and timing of puberty
Insulin-like peptide 3 (INSL3) is a major regulator of female reproductive physiology
Testicular function and bone in young men with severe childhood-onset obesity.