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A fluorescent hormone biosensor reveals the dynamics of jasmonate signalling in plants

Larrieu, Antoine; Champion, Antony; Legrand, Jonathan; Lavenus, Julien; Mast, David; Brunoud, Geraldine; Oh, Jaesung; Guyomarc’h, Soazig; Pizot, Maxime; Farmer, Edward E.; Turnbull, Colin; Vernoux, Teva; Bennett, Malcolm J.; Laplaze, Laurent

Authors

Antoine Larrieu

Antony Champion

Jonathan Legrand

Julien Lavenus

David Mast

Geraldine Brunoud

Jaesung Oh

Soazig Guyomarc’h

Maxime Pizot

Edward E. Farmer

Colin Turnbull

Teva Vernoux

Laurent Laplaze



Abstract

Activated forms of jasmonic acid (JA) are central signals coordinating plant responses to stresses, yet tools to analyse their spatial and temporal distribution are lacking. Here we describe a JA perception biosensor termed Jas9-VENUS that allows the quantification of dynamic changes in JA distribution in response to stress with high spatiotemporal sensitivity. We show that Jas9-VENUS abundance is dependent on bioactive JA isoforms, the COI1 co-receptor, a functional Jas motif and proteasome activity. We demonstrate the utility of Jas9-VENUS to analyse responses to JA in planta at a cellular scale, both quantitatively and dynamically. This included using Jas9-VENUS to determine the cotyledon-to-root JA signal velocities on wounding, revealing two distinct phases of JA activity in the root. Our results demonstrate the value of developing quantitative sensors such as Jas9-VENUS to provide high-resolution spatiotemporal data about hormone distribution in response to plant abiotic and biotic stresses.

Journal Article Type Article
Publication Date Jan 1, 2015
Journal Nature Communications
Electronic ISSN 2041-1723
Publisher Nature Publishing Group
Peer Reviewed Peer Reviewed
Volume 6
Issue 1
APA6 Citation Larrieu, A., Champion, A., Legrand, J., Lavenus, J., Mast, D., Brunoud, G., …Laplaze, L. (2015). A fluorescent hormone biosensor reveals the dynamics of jasmonate signalling in plants. Nature Communications, 6(1), https://doi.org/10.1038/ncomms7043
DOI https://doi.org/10.1038/ncomms7043
Keywords Arabidopsis, jasmonate, jasminic acid, biosensor, signalling
Publisher URL http://www.nature.com/ncomms/2015/150116/ncomms7043/full/ncomms7043.html
Related Public URLs https://www.cpib.ac.uk/people/malcolm-bennett/
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0





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